用CellTrace Violet(CTV)标记4-羟基-3-硝基苯基乙酰基(NP)特异性B1-8hi AicdaGFP/+B细胞,并将其过继转移到小鼠体内。由于敲除B1-8hi重链与Igl配对产生NP的高亲和力受体,他们在免疫后7天检测了Igλ+B1-8hi CD45.1+细胞的表型。GL7在第一轮细胞分裂中上调。原始标记物CD38和IgD的丢失以及相应的GC标记物CD95...
GL7+B220low BM cells have a large cell size and are CD43–to low, CD95–, Sca-1–, I-Alow, IgM– and IgD–, suggesting that they are large pre-B cells. These BM cells express λ5 and VpreB but notterminal deoxytransferase (TdT) and Bcl-2, and approximately 50 % of ...
The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc. Cross-reactivity to ferret has been reported by a collaborator, but not verified in house. Application References (PubMed link indicates BioLegend citation) ...
The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc. Cross-reactivity to ferret has been reported by a collaborator, but not verified in house. Application References (PubMed link indicates BioLegend citation) ...