160 40 U/μl Taq ligase (NEB) 700 μl ddH2O 混合均匀后分装到PCR管中 (15 μl/管) 并保存在-20℃冰箱 参考文献: [1] Gibson, D.G., et al. (2009) Nat. Methods 6, 343–345. doi.org/10.1038/nmeth.1 [2] Gibson, D.G., et al. (2008) Science 319, 1215–1220. doi.org/10....