GeneArt Gibson Assembly HiFi 克隆试剂盒(含电转感受态细胞)是包括预混液、阳性对照,水和 ElectroMAX DH10B 电转感受态大肠杆菌的全套试剂盒。GeneArt Gibson Assembly HiFi 克隆试剂盒的特点包括:•简便—在单次反应中可无缝组装和克隆多达 6 个 DNA 片段•高效—高保真度可提供比其他方法更多的正确克隆•...
1.设计引物以扩增具有适当重叠的片段(和/或载体); 2.制备线性化载体(使用高保真DNA聚合酶扩增片段通过使用高保真度DNA聚合物或限制性内切酶消化); 3.将片段和线性化载体添加到NEBuilder HiFi DNA Assembly Master Mix中,并在50°C下孵育15分钟到1小时,具体取决于组装的片段数量。 4.转化至大肠杆菌感受态或直接用于...
When using a polymerase that doesn’t contain a 3′-5′ exonuclease activity (such as Taq DNA Polymerase) to amplify fragments to be used in a Gibson Assembly reaction, should I be concerned about the potential 3′ mismatch generated by the addition of a non-templated nucleotide? When my G...
GeneArt Gibson Assembly HiFi 方法具有多功能性,可通过快速组合、添加、删除或交换 DNA 片段来简化许多技术,且无需亚克隆,从而节省了克隆工作流程的时间和精力。 便利的规格 GeneArt Gibson Assembly HiFi 试剂盒有多种规格可供选择,包括: • GeneArt Gibson Assembly HiFi 克隆试剂盒,含化学感受态细胞(含 One ...
Gibson Assembly®(也叫NEBuilder® HiFi DNA Assembly Cloning Kit) 反应体系 首先,我们来说说反应体系的配置。这个过程需要在冰上进行哦! 2-3片段组装:推荐使用50–100 ng的载体,配合每个插入片段的2-3倍摩尔过量。对于小于200 bp的插入片段,建议使用5倍摩尔过量(载体通常为100 ng)。 4-6片段组装:每个插入...
Gibson Assembly Cloning(同源重组kit) 英文名称:总访问:288 国产/进口:国产半年访问:22 产地/品牌:AOGMA产品类别:分子生物学试剂 规格:100rxns最后更新:2025-3-19 货号:AGM90229L CAS 号: 参考报价:5176 立即询价电话咨询 [发表评论][本类其他产品][本类其他供应商][收藏] ...
but it is also useful for more routine applications such as cloning. The Gibson Assembly cloning kit which includes both Gibson Assembly Master Mix and NEB® 5-alpha competent cells, has been optimized for efficient assembly and cloning of multiple fragments into any vector in just over an ...
Gibson Assembly® HiFi kit Gibson Assembly® master mix (2X)Important: Upon receipt, place Gibson Assembly master mix (2X) on ice to thaw. Briefly vortex and centrifuge the thawed master mix. Then, aliquot the master mix to reduce the number of freeze-thaw cycles. Properly aliquoted Gibson...
Gibson Assembly ® HiFi 1-Step Kit Quick Reference Manual Complete product information and additional resources are available at www.sgidna.com/hifi_kit .Catalog Numbers GA1100-10, GA1100-50, GA1100-S, GA1100-10MM, GA1100-50MM Products and Storage Conditions Gibson Assembly ® HiFi 1 Step...
DNA MODIFYING ENZYMES Gibson Assembly ™ Cloning KitAgindotan, BrightPerry, Keith L