The C-terminal 6×His tag of the Ni-NTA eluent was cleaved by 3C protease and further purified by gel filtration chromatography using a Superdex 200 column. Finally, the purified scFv16 was concentrated and stored at −80 °C until further use. Expression and purification of heterotrimeric...
For those of you who know us and have been a part of this ministry, you may have heard that our book{Dancing On My Ashes: learning to love the One who gives and takes away}has been unavailable. Our original publisher went out of business, which threw us for a loop… BUT we clung ...
marine drugs Article Alanine-Scanning Mutagenesis of α-Conotoxin GI Reveals the Residues Crucial for Activity at the Muscle Acetylcholine Receptor Jiong Ning, Rui Li, Jie Ren, Dongting Zhangsun, Xiaopeng Zhu, Yong Wu * and Sulan Luo * Key Laboratory of Tropical Biological Resources, Ministry of...
Aside from the hefty price tag they are perfect machines. I own several Kubota combine harvesters, but I would like some more." Mr. Dong receives payment to harvest about 1,000 ha of farmland each year, which requires him to run his combine harvesters for several hundred hours annually (...