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All experimental procedures were approved by the Institutional Animal Care and Use Committee of Shanghai University. Cell culture The human skin keratinocytes (HaCaT cells) were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and the human umbilical vein ...
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After washing with TBST 3 times, the membranes were detected with the BeyoECL Star kit (Beyotime Biotechnology, Shanghai, China) and placed in the gel imaging system (Uvitec, Cambridge, Britain). Images were analyzed by measuring the intensity of correctly sized bands using Alpha Imager 2200 (...
24 SLP was obtained from LGG using ultrafiltration with 5M LiCl.25 EPSs were extracted using trichloroacetic acid and ethanol according to previous methods.26 The CpG-ODN 5′-ACTTTCGTTTTCTGCGTCAA-3′ was synthesized by Sangon Inc. (Shanghai, China). LGG immunomodulation of LPS-stimulated IPEC-...
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