利用分子生物学技术成功构建了重组表达载体pLVX-AcGFP1-N1-TFEB,并进行慢病毒包装,进而侵染HeLa细胞,通过嘌呤霉素筛选阳性单克隆细胞株.采用荧光显微镜技术及免疫印迹技术(Western blot)证实稳定表达TFEB-GFP细胞株构建成功.利用该细胞模型筛选得到促TFEB入核药物盐酸舍曲林(Sertraline HCl),此药物能够促进...
本研究旨在构建稳定表达TFEB-GFP的HeLa细胞系,并利用其筛选促进转录因子EB(TFEB)入核药物.利用分子生物学技术成功构建了重组表达载体pLVX-AcGFP1-N1-TFEB,并进行慢病毒包装,进而侵染HeLa细胞,通过嘌呤霉素筛选阳性单克隆细胞株.采用荧光显微镜技术及免疫印迹技术(Western blot)证实稳定表达TFEB-GFP细胞株构建成功.利用该...
Chinn H, Gardell J, Matsumoto L, Labadie K, Mihailovic T, Lieberman N,et al. Hypoxia-inducible lentiviral gene expression in engineered human macrophages. J Immunother Cancer. 2022;10:pubmedpublisher Dang T, Back S. Translation Inhibitors Activate Autophagy Master Regulators TFEB and TFE3. Int ...
Print datasheet MSDS ¥980/100 μL Cat No.50430-2-AP 浓度:1000 ug/ml 库存:现货 Size mouse,hamsterWB Programmable protein expression using a genetically encoded m6A sensor See more citations
A SPLICS reporter reveals \({{{\boldsymbol{\alpha }}}\)-synuclein regulation of lysosome-mitochondria contacts which affects TFEB nuclear translocation Flavia Giamogante Lucia Barazzuol Marisa Brini Nature Communications (2024) Perturbation of the host cell Ca2+ homeostasis and ER-mitochondria...
(ortholog of CCK); CTSB: cathepsin B; CTSD: cathepsin D; CTSL: cathepsin L; ER: endoplasmic reticulum; LAMP: lysosomal-associated membrane protein; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; TEM: transmission electron microscopy; TFEB: transcription factor EB; ZG: zymogen ...
pCellFree_G03 TFEBpCellFree_G03 GTF2A1LpCellFree_G03 SMAD2pCellFree_G03 GTF2H4pCellFree_G03 GDI2pCellFree_G03 TFAP2BpCellFree_G03 RUVBL1pCellFree_G03 CYP2D6pCellFree_G03 GTF2E1pCellFree_G03 TFDP2pCellFree_G03 PSMC1pCellFree_G03 CALRpCellFree_G03 CSNK2A1pCellFree_G03 TFDP1pCell...
In vivo pancreatitis caused autophagy impairment in WT mice, manifest by upregulation of LC3-II and SQSTM1/p62, increased number and size of autophagic vacuoles, and decreased level of TFEB, all of which were exacerbated in GFP-LC3 mice. GFP-LC3 expression affected key pancreatitis responses; ...