List of primers used for RT-qPCR and GFP fusion constructs.Pai, PedasMichaela, Schiller StokholmJosefine, Nymark HegelundAnne, Hald LadegårdJan, Kofod SchjoerringSøren, Husted
In order to assess specificity of the observed off-target effect, we transfected increasing amounts of the siRNA directed against GFP and measured expression of CYLD and SOAT as off-target genes and of C13ORF1 and LMNB1 as non-regulated, negative controls using qPCR (Figure 3a, b). In add...
AIL-18 mRNA was detected by qRT-PCR.BIL-18 protein was detected by western blotting.CThe secretion of IL-18 was detected.DThe binding site of YBX1 in the JASPAR database was showed and the binding sites in IL-18 promoter was showed. P1-6 showed 6 paired primers that covered the 8 ...
The amount ofsfGFP,GFP1-10andGFP1-10(TGA11)transcripts was analyzed by RT-qPCR as described previously [43]. RNA was isolated in three steps from 500 µL expression culture using the NucleoSpin®RNA Kit (Macherey–Nagel, Düren, Germany), the RNase-Free DNase Set (Qiagen, Hilden, Ger...
Quantitative real-time PCR (qPCR) was carried out in a CFX384 Real-Time System (Bio-Rad, USA). To analyze the expression of target genes and virus genome, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as reference gene. The primers used to amplify target and reference genes are...
(SST), persist under challenging environmental conditions. The present study explored the changes in the symbiotic dinoflagellate and GFP-like proteins in a heat stress tolerant submassivePoritescorals versus heat stress sensitive tabularAcroporacorals across seasonal and depth gradients in Kish Island in...
(RT) into cDNA. Gene expression was evaluated via PCR using primers specific for the four factors and a Gapdh loading control. Only the cells co-transfected with the PiggyBac Mouse 4-in-1 iPSC Vector showed expression of the transcripts. (B). Cells from (A) were also imaged for ...
The qPCR cycling parameters were: 1 cycle of 95°C for 10 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. Data collection was taken at the 60°C annealing/extension phase. In order to ensure the presence of a single product, a dissociation curve was performed after each ...
The qPCR cycling parameters were: 1 cycle of 95°C for 10 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. Data collection was taken at the 60°C annealing/extension phase. In order to ensure the presence of a single product, a dissociation curve was performed after each ...