该GFP Monoclonal Antibody. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) ...
背景太高。Proteins bind nonspecifically to the Anti-GFP monoclonal antibody,insufficient washing on magnetic beads,or the microcentrifuge tubes.1.Pre-clear lysate with Mouse IgG Magnetic Beads (P2171) to remove nonspecific binding proteins.
背景太高。 Proteins bind nonspecifically to the Anti-GFP monoclonal antibody,insufficient washing on magnetic beads,or the microcentrifuge tubes. 1.Pre-clear lysate with Mouse IgG Magnetic Beads (P2171) to remove nonspecific binding proteins. 2.After suspending beads for the final wash,transfer enti...
具体方法见已公开文献:Ran Zhuang, Yuan Zhang, Rui Zhang, Chaojun Song, Kun Yang, Angang Yang, Boquan Jin.Purification of GFP fusion proteins with high purity and yield by monoclonal antibody-coupled affinity column chromatography. Protein Expression and Purification. 2008 ;59(1) : 138-143,本...
Name GFP Mouse Monoclonal Antibody Category Monoclonal antibody(mAb); Primary antibody Isotype IgG1 Purification ProA affinity purified About the Immunogen Immunogen This antibody is produced by immunizing mice with a recombinant protein corresponding to full length GFP. Gene ID 7011691 SwissProt P42212...
無性繁殖 GSN24, monoclonal 形狀 buffered aqueous solution 分子量 antigen 27 kDa 濃度 ~2 mg/mL 技術 western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells 同型 IgG1展开 说明 一般說明 绿色荧光蛋白 (GFP) N端抗体(小鼠单抗,IgG1同种型)来源于小鼠骨髓瘤...
GFP-Tag Rabbit monoclonal antibody 英文名:GFP-Tag Rabbit monoclonal antibody 储存条件:-20℃ Product Name Anti-GFP (Green fluorescent protein) polyclonal antibody Immunogen: Recombinant GFP expressed in E.coli Purification: antigen affinity purification ...
Class MonoclonalType AntibodyFull Name GFP tagSynonyms CFP, eGFP, eYFP, GFP, GFP tag, GFP-tag, YFPCalculated Molecular Weight 26 kDaGenBank Accession Number U73901Gene Symbol Gene ID (NCBI) RRID AB_11182611Conjugate UnconjugatedForm LiquidPurification Method Protein A purificationStorage Buffer PBS ...
Affinity purificationGFPHigh purityMonoclonal antibodyGFP has often been used as a marker of gene expression, protein localization in living and fixed tissues as well as for protein targeting in intact cells and organisms. Monitoring foreign protein expression via GFP fusion is also very appealing for...
NameGFP Mouse Monoclonal Antibody CategoryMonoclonal antibody(mAb); Primary antibody IsotypeIgG1 PurificationProA affinity purified About the Immunogen ImmunogenThis antibody is produced by immunizing mice with a recombinant protein corresponding to full length GFP. ...