GFP labeled neurons in GAD67-GFP BAC transgenic mice are GABAergic neurons.Shengli, Zhao
We generated transgenic mice in which red, green, yellow, or cyan fluorescent proteins (together termed XFPs) were selectively expressed in neurons. All four XFPs labeled neurons in their entirety, including axons, nerve terminals, dendrites, and dendritic spines. Remarkably, each of 25 independent...
Immunostaining: anti-GFP (green); anti-NeuN for neurons (red), DAPI for nuclei (blue). Confocal microscopy imaging of the Thy1-GFP transgenic rat brain. To verify the GFP transgene expression and analyze its pattern, the rat brains were cut into sections and stained with antibodies. ...
labeled with GFP Polyclonal Antibody (Product # A-11122) at 1:200 dilution and Histone H3 Monoclonal Antibody (865R2) Product # AHO1432) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed ...
Knock-in mice with GABAergic neurons labeled by expression of green fluorescent protein (GFP) under control of the glutamate decarboxylase 67 (GAD67) promoter are now extensively used in neuroscience. However, it is unknown whether these mice have a normal sleep phenotype. Compared with wild-type...
Then single cell clones of MSCs labeled with GFP under optimal condition were selected by using cloning rings. Results The efficiency of infection for 24h at MOI 100, 200 and 400 was 88.94%, 99.65% and 99.42%, respectively. The infection had no significant effect on the proliferation of MSCs...
labeling for Fos (the protein product of the immediate early gene c-fos) was used to identify and quantify Fos expressed in dorsal and ventral mPFC neurons during the reinstatement tests. Sections were thawed and washed 3 ϫ 10 min in PBS, incubated for 1 h in 3% normal goat serum (NGS...
Generation of dopaminergic neurons in the adult brain from mesencephalic precursor cells labeled with a nestin-GFP transgene. J. Neurosci. 21, 3895-3903.Sawamoto K,Nakao N,Kakishita K,et al.Generation of dopaminergic neurons in the adult brain from mesencephalic precursor cells labeled with a ...
neurons project to the striatum.During the process of emb巧omcdevelopment the neurominthemidbrain graduallyproject tothestriatum anddifferentiate into functional dopaminergic neuronsinduced by the striatal microenvironment.Studieshaveshownthat the striatumcontains a ...
We use live-cell imaging and RNAi in primary neurons from GFP-LC3 transgenic mice to show that htt and HAP1 control autophagosome dynamics, regulating ... YC Wong,ELF Holzbaur - 《Journal of Neuroscience the Official Journal of the Society for Neuroscience》 被引量: 171发表: 2014年 ...