Proliferation markers colocalize with GFP and luciferase proteins in MITO-Luc/GFP zebrafish tissues.(A) Immunofluorescence analysis of DAPI (I), pH3 (II), GFP (III), HuC/D(IV), DAPI-pH3 merge (V), pH3-HuC/D merge (VI), pH3-GFP merge (VII) and GFP-HuC/D merge (VIII) in the...
MT polymerizing activity preferentially located at the periphery of the aster. (J–L) High resolution CLSM imaging of a YCL aster in a dclk2-GFP embryo, fixed and stained for DAPI (blue) at 60% epiboly. (J) corresponds to yellow inset in (L). No chromatin enriched structures are detect...
For subcellular localization experiments, cells were imaged using LSM 880 (Zeiss, Oberkochen, Germany) confocal laser scanning microscope, using a 488 Argon laser with a 490–553 nm emission filter for GFP; Alexa 647 and DAPI labelling were acquired with Diode Pumped Solid State Lasers (DPSS)...
(β1/α-HA merge) reconstitutes the triFC ternary complex (green biofluorescence). Scale bar: 10 µm. (B) T10HA-TDP-43 and T11β1-TDP-43 (just above the 55 kDa marker) were also detected in RIPA lysates of cells transfected with the indicated plasmids by duplex-western blot ...