When absorbance at 600 nm value was 0.7, isopropyL -D-thiogalactopyranos 6、ide was added to 0.8 mM and the incubation was continued an addition 3 h.Key words: Green fluorescent protein Fellyfish Genetic Cloning Fluorescin目录1.前言: . 1 III错误!未指定书签。1.1绿色荧光蛋白(GREEN FLUORESCENT...
When absorbance at 600 nm value was 0.7, isopropyL β-D-thiogalactopyranoside was added to 0.8 mM and the incubation was continued an addition 3 h. Key words:Green fluorescent protein Fellyfish Genetic Cloning Fluorescin 前言: 1.1绿色荧光蛋白(green fluorescent protein,GFP) 1.1.1 GFP研究背景 ...
Figure 1. Transfected GFP is detected by PathScan ® Total GFP Sandwich ELISA Kit #7878. The relationship between the protein concentration of the lysates from untreated and GFP-transfected HEK293 cells and the absorbance at 450 nm is shown in the top figure, while the corresponding Western bl...
To be effective, the dye should have an absorbance spec- trum that significantly overlaps the auto- fluorescence emission. The dye also should have a low molecular weight and high solubility so that it rapidly and evenly diffuses throughout the sample. And fi- nally, it should have low ...
An Absorbance-Based Assay for Cell Health and Proliferation Multiparametric Flow Cytometry Assays for Determining Pharmacological Effects Flow Cytometry Compensation Tools for a Host of GFP Variants Evaluation of Different Techniques f...
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm. Alternative names GFP antibody Green Fluorescent Protein antibody yfp antibody Anti-GFP antibody images Immunofluorescence - GFP antibody (ab6556) This image shows IF using...
Absorbance data from analytical ultra-centrifugation for mRFP, GFP and Dual PI.Sebastian Evesvan den AkkerCatherine J. LilleyJames R. AultAlison E. AshcroftJohn T. JonesPeter E. Urwin
Item No.600-305-215 Size:1 mg Physical State:Liquid (sterile filtered) Applications:WB, ELISA, Biochemical Assay Reactivity:GFP, eGFP, rGFP Host Species:Mouse $1,107.00/Per Item In stock Ships next business day Add To Cart Add to List ...
One can observe acylimine reactivity upon protein denaturation, when H2O addition across the acylimine C=N bond interrupts the extra conjugation of a DsRed-like chromophore and reverts its red-shifted spectra back to a GFP-like absorbance (Gross et al., 2000). However, compared with simple ...
It was found that the absorbance and fluorescence of green fluorescent protein (GFP) mutants are strongly pH dependent in aqueous solutions and intracellul... M Kneen,J Farinas,Y Li,... - 《Biophysical Journal》 被引量: 978发表: 1998年 High-voltage electroporation of bacteria: genetic transf...