GFAP启动子介导EGFP靶向肝星状细胞治疗 肝纤维化的价值探索 范建华 1 ,徐曼妮 2 △ (1.上海市黄浦区中心医院消化内科 200002;2.同济大学医学院预防医学 教研室,上海 200092) 【摘要】 目的 探索体内特异性靶向肝星状细胞(HSC)治疗肝纤维化的技术方法。方法 建立四氯化碳诱导 的小鼠肝纤维化模型,构建胶质纤维酸性...
目的探索体内特异性靶向肝星状细胞(HSC)治疗肝纤维化的技术方法。方法建立四氯化碳诱导的小鼠肝纤维化模型,构建胶质纤维酸性蛋白(GFAP)启动子调控增强绿色荧光蛋白(EGFP)表达载体,采用尾静脉高压注射方法将EGFP表达裸质粒转染到纤维化小鼠的肝脏。荧光显微镜观察EGFP在肝脏的表达及免疫荧光共定位,观测EGFP与HSC标志蛋白(...
摘要:目的 构建并鉴定带GFAP启动子的Flag-p27和EGFP双顺反子真核表达载体,观察其表达。方法 利用IRES和GFAP启动子,通过质粒抽提、琼脂糖凝胶电泳、酶切、连接、转化等多种基因工程技术,经多步亚克隆后完成能同时表达p27和EGFP基因的星形胶质细胞特异性真核表达载体pGFAP-IRES2-EGFP-p27。转染体外培养的星形胶质细...
Employing single-cell RT-qPCR, we detected the expression of genes encoding subunits of glutamate receptors in GFAP / EGFP -positive ( GFAP / EGFP + ) glial cells in the cortex of young adult mice. Most of the analyzed cells expressed mRNA for glutamate receptor subunits, the expression of ...
寻标宝于2024-11-22发布中山医学院LV-GFAP(2.2Kb)-neurogenin2-GSG-P2A-EGFP-WPRE,项目位于广东省,项目编号null。寻标宝为您提供广东省2024年最新的招标采购信息查询服务。
eGFP-GFAPOligodendrocytes, the myelinating cells of the central nervous system, orchestrate several key cellular functions in the brain and spinal cord, including axon insulation, energy transfer to neurons, and, eventually, modulation of immune responses. There is growing interest for obtaining ...
目的 构建并鉴定带GFAP启动子的Flag-p27和EGFP双顺反子真核表达载体,观察其表达.方法 利用IRES和GFAP启动子,通过质粒抽提,琼脂糖凝胶电泳,酶切,连接,转化等多种基因工程技术,经多步亚克隆后完成能同时表达p27和EGFP基因的星形胶质细胞特异性真核表达载体pGFAP-IRES2-EGFP-p27.转染体外培养的星形胶质细胞,观察...
In this study, we used transgenic mice in which astrocytes are labeled by the enhanced green fluorescent protein (eGFP) under the control of the human glial fibrillary acidic protein (GFAP) promoter. By detailed co-localization studies we were able to demonstrate that a significant proportion of ...
High potassium exposure reveals the altered ability of astrocytes to regulate their volume in the aged hippocampus of GFAP/EGFP miceAstrocyteVolume regulationHippocampusPotassium/glutamate uptakeAgingIn this study, we focused on age-related changes in astrocyte functioning, predominantly on the ability of ...
Gene expression profile of cortical astrocytes from GFAP/EGFP and GFAP/EGFP/α-Syn−/− mice.Miroslava AnderovaJana BenesovaMichaela MikesovaDavid DzambaPavel HonsaJan KriskaOlena ButenkoVendula NovosadovaLukás ValihrachMikael Kubista