方法 hG fapCreERT2/Rosa26R转基因小鼠在胚胎晚期和出生早期用他莫昔芬诱导Cre重组酶表达,对小脑组织切片行X- ga1染色,然后用细胞种类特异性抗体进行免疫组织化学染色,并和X- ga1染色双重标记。结果 在出生后第7天( P7) 、第14天( P14)和第60天( P60) ,X- ga1阳性染色和胶质细胞抗体Bl bp阳性染... ...
Gfap 人类同源基因 GFAP 品系描述 将IRES-CreERT2插入到大鼠Gfap基因终止密码子处。 *使用本品系发表的文献需注明: Gfap-IRES-CreERT2(SD) rats (Cat. NO. NR-KI-241320) were purchased from Shanghai Model Organisms Center, Inc.. 相关品系
Methods : We utilize of a model of hypoxia-induced retinopathy to model the ischemia and neovascularization of DR. The GFAP-CreERT2:Rosa26iDTR mouse line renders astrocytes sensitive to diphtheria toxin (DTx) following tamoxifen administration and induces astrocyte hypertrophy. To test for astrocyte ...
Pharmacological inhibition studies revealed that Notch1 signaling is necessary to maintain NSC identity, whereas serum treatment induced cell differentiation into reactive astrocytes and neurons.Collectively, these results indicate that GFAP promoter-driven porcine CreER T2 NSCs would be a useful tool to ...
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...
To investigate the role of astrocytes in hypoxia-induced retinopathy, we utilized a 7-day systemic hypoxia model using the GFAP-CreERT2:Rosa26iDTR transgenic mouse line. This allows for the induction of inflammatory reactive astrogliosis following tamoxifen and diphtheria toxin administration. We ...