The Gesicle Producer 293T Cell Line is not provided with the system and may be purchased separately (Cat. # 632617) Stem cell applications: We have demonstrated high-efficiency editing of endogenous genes in human iPS cells using a combination of CRISPR/Cas9 gesicles and our DEF-CS culture sy...
Interestingly, the proteins packaged into gesicles may be structurally different than in the producer cell line. For example, the cherry fluorescent protein is expressed as an ∼30-kDa monomer,47 and addition of the DmrA domain to form CherryPicker Red is ∼60 kDa. We observed four ...