Here, phenotype-genotype mapping was used to dissect the betaine-triggered L-arginine overproduction mechanism and mine beneficial genes for further improving production phenotype. The comparative transcriptomic analysis revealed a novel role for betaine in modulating global gene transcription. Guided by ...
As we look forward to 2017, Cell Systems expects that advances in technology will redefine an age-old problem: the mapping of phenotype onto genotype. Several landmark papers published in recent weeks describe experimental methods to address this problem—Perturb-seq (Dixit et al., Cell 167, 18...
- git clone https://github.com/SakshiKhaiwal/Genotype-to-phenotype-mapping-in-yeast.git - cd Genotype-to-phenotype-mapping-in-yeast/pipeline - conda create -n GenPhen python=3.8 - conda activate GenPhen - pip install -r requirements.txt ...
Microbial phenotypes are typically due to the concerted action of multiple gene functions, yet the presence of each gene may have only a weak correlation with the observed phenotype. Hence, it may be more appropriate to examine co-occurrence between sets of genes and a phenotype (multiple-to-on...
Firstinsightsintothegenotype–phenotypemapo phenotypicstabilityin rye Yu Wang 1,2 ,Michael FlorianMette 1 ,Thomas Miedaner 2 ,Peer Wilde 3 ,JochenC. Rei 1, *andYusheng Zhao 1 1 Departmento BreedingResearch,LeibnizInstituteo PlantGeneticsandCropPlantResearch(IPK),D-06466Gatersleben, ...
Genome-scale engineering is an indispensable tool to understand genome functions due to our limited knowledge of cellular networks. Unfortunately, most existing methods for genome-wide genotype–phenotype mapping are limited to a single mode of genomic a
The pleiotropic structure of the genotype–phenotype map is strongly modular, meaning that sets of traits are co-controlled by sets of genes in a highly nonrandom manner. In contrast to current theoretical models of pleiotropy, empirical data show that the average phenotypic effect of a mutation ...
Genome-scale engineering is an indispensable tool to understand genome functions due to our limited knowledge of cellular networks. Unfortunately, most existing methods for genome-wide genotype–phenotype mapping are limited to a single mode of genomic alteration, i.e. overexpression, repression, or de...
Genome Biology (2023) 24:196 https://doi.org/10.1186/s13059-023-03044-2 Genome Biology RESEARCH Open Access A high‑resolution genotype–phenotype map identifies the TaSPL17 controlling grain number and size in wheat Yangyang Liu1,2†, Jun Chen3†, Changbin Yin4†, Zi...
These findings illustrate the power of genome-wide reverse genetics in genotype-phenotype mapping, uncover an enormous range of genetic complexity of phenotypic traits, and suggest that the GPM of cellular organisms has been shaped by natural selection for mutational robustness. © The Author ...