Colibactin alkylates double-stranded DNA, causing inter-strand crosslinks, double-strand breaks (DSB), and a specific mutational signature commonly found in CRC. Despite the growing knowledge about colibactin synthesis and its mode of action, the mechanism by which it is delivered from bacteria ...
Yih-Ru Shiau7, Hui-Ying Wang7, Ann-Chi Lin1, Jui-Fen Lai7, I-Wen Huang7 & Tsai-Ling Lauderdale7 The pks gene cluster encodes enzymes responsible for the synthesis of colibactin, a genotoxin that has been shown to induce DNA damage and contribute to increased virulence. ...
Inulin impacts tumorigenesis promotion by colibactin-producing Escherichia coli in ApcMin/ mice (pks+) Escherichia coli promotes carcinogenesis and facilitates CRC progression through the production of colibactin, a genotoxin that induces double-strand ... M Oliero,R Hajjar,T Cuisiniere,... - 《...
Genotoxins are agents or chemicals that cause DNA or chromosomal damage, thereby causing mutations. The discovery of colibactin in 20061 verified that the vast metabolic potential of the human gut ...
β-actin was used as loading control. Lower panel shows an immunoblot of total kidney extract from wild-type mice loaded as 2-fold dilution series, comparing the relative expression of ADH5 with bone marrow. (C) Formaldehyde reacts with guanine to form the N2-hydroxymethyl-dG adduct, which ...
The bacterial genotoxin colibactin promotes colon tumor growth by modifying the tumor microenvironment[J] . Guillaume Dalmasso,Antony Cougnoux,Julien Delmas,Arlette Darfeuille-Michaud,Richard Bonnet.Gut Microbes . 2014 (5)Dalmasso G, Cougnoux A, Delmas J, et al. The bacterial genotoxin co-lib...
Bacterial genotoxin colibactin promotes colon tumour growth by inducing a senescence-associated secretory phenotypeE.ColiColonCarcinogenesisGeneExpressionDNADamageCellProliferationBackground Escherichia coli strains harbouring the pks island (pks+ E. coli) are often seen in human colorectal tumours and have ...
MATE transport of the E. coli-derived genotoxin colibactinVarious forms of cancer have been linked to the carcinogenic activities of microorganisms1,2,3. The virulent gene island polyketide synthase (pks) produces the secondary metabolite colibactin, a genotoxic molecule(s) causing double-...
A similar DNA-damaging mechanism is observedin cellulo, where interstrand cross-links are detected in the genomic DNA of cultured human cells exposed to colibactin-producing bacteria. The intoxicated cells exhibit replication stress, activation of ataxia-telangiectasia and Rad3-related kinase (ATR), ...
In eukaryotic cells, colibactin can induce DNA damage, cell cycle arrest and chromosomal instability. Production of colibactin has been implicated in the development of colorectal cancer (CRC). In this study, we demonstrate the inhibitory effect of D-Serine on the expression of the pks island ...