The direct use of purified genomic DNA as a template for cycle sequencing is new and the DNA is purified by phenol-chloroform extraction or by chromatography.doi:DE19740795PESCHEL ANDREAS DRTHUMM GUENTHER DROTTO MICHAELGOETZ FRIEDRICH PROF DR...
DNA nanowireselectron microscopyUV spectroscopyIt has been previously found that in a PCR with yeast genomic DNA as a template, microparticles of condensed DNA are formed in the presence of KlenTaq polymerase. In the present work, the study of these microparticles was continued using electron ...
athe template (Qiagen, MD). Several deletion Adam21-Luc reporter plasmids were also prepared using the same reverse primer and 模板(Qiagen, MD)。 几删除Adam21-Luc记者质粒使用同一反向底漆也准备了和 [translate] aconstructs. The human genomic DNA (Clontech, CA) was used as the template of the...
Through a genome-wide analysis of DNA methylation across 19 cell types with T-47D as reference, we identified 106,252 cell type-specific differentially-methylated CpGs categorized into 7,537 differentially (46.6% hyper- and 53.4% hypo-) methylated regions. We found 44% promoter regions and 75%...
The DNA was extracted from faeces samples in one month, five months later and eight months later, respectively. The extraction of DNA as a template was used for PCR, gene cloning and sequencing. Results The results showed that DNA templates were extracted successfully from the faeces in a ...
The genomic DNA extracted by advanced CTAB method from the fresh leaves of wheat seedlings,which could be used for RAPD analysis as template.During the research,the main parameters in polymerase chain reaction were studied by setting gradient in order to obtain an optimal system for RAPD analysis...
Single-base extension of the oligos on the BeadChip, using the captured DNA as a template was performed. The bead chips were then imaged using an Illumina HiScan. DNA array data was processed using Illumina Genome Studio to compute the log2R ratio (LRR) and the B-allele frequency (BAF)....
A 10 μl aliquot of each PCR reaction was separated on an agarose gel. The comparative expression analysis of ThSOS1 and ThSOS1S was performed by quantitative PCR with LightCycler Systems for Real-Time PCR (Roche Applied Science, Japan) using the LightCycler-FastStart DNA Master SYBR Green I...
The remaining sequences were similar to nuclear genes in GenBank (5%), mitochondrial DNA (4%), plastid DNA (1%) or ncRNAs, repetitive elements and sequences annotated as genomic markers (2%). Finally, 2,916 contigs, equal to ~9% of the assembled transcriptome, had no significant match ...
sequence (CG14) or a control B-DNA-forming sequence was inserted into the YAC adjacent to theURA3gene18,19, where the structure-induced chromosomal breakage results in loss ofURA3, as assessed by 5-Fluoroorotic acid resistance (FOAR) (Fig.1b). We found that the 28-bp Z-DNA-forming ...