Moreira D, Rodriguez-Valera F, Lopez-Garcia P. 2004. Analysis of a genome fragment of a deep-sea uncultivated Group II euryarchaeote containing 16S rDNA, a spectinomycin-like operon and several energy metabolism genes. Environ Microbiol. 6:959-969....
Targeted sequencing of genetic regions that differ between two DNA preparations uses genomic fragment enrichment. This method can be used to study genetic variation among closely related species and m
A fragment (3 336 bp) of LsNPV genome has been sequenced. It contains three complete ORFs (ORF2, ORF3, ORF4) and two incomplete ORFs (ORF1, ORF5). Comparing with AcNPV genome it was found that ORF1 coding product was homologous with P94 protein ORF3, ORF4 and ORF5 were homologou...
软枣猕猴桃是猕猴桃属内倍性最复杂的物种之一,本研究针对分布在国内4个省份具有代表性的7个野生软枣猕猴桃居群,选用21对叶绿体基因组高变片段对7个居群的软枣猕猴桃进行序列比对、系统构建和多态性分析。研究发现,21对引物中有7对引物在7个软枣猕猴桃居群样本中均能扩增
Long fragment editing techniques are of great importance for accelerating bacterial genome engineering to obtain desirable and genetically stable strains. However, the existing genome editing methods cannot meet the needs of engineers. We herein report an efficient long fragment editing method for large-...
Here, we present a traceable and efficient PCR strategy: randomly broken fragment PCR with 5′ end-directed adaptor for genome walking. The genome is first fragmented randomly. After blunting ends, the fragments are ligated to the 5′ end-directed adaptors. Semi-nested PCR is then performed. ...
Mapping the Human Genome Using Restriction Fragment Length PolymorphismsIt is a truism that people, like all living organisms, are the products of their heredity, their environmental history, and the interaction between their heredity and their environment. This truism applies, of course, to any ...
Cloning and Characterization of a Repetitive 1.9 Kb HindIII DNA Fragment from Crotalus durissus terrificus Genome 来自 Semantic Scholar 喜欢 0 阅读量: 21 作者:Margaret,H.,Magdesian,and,lvaro,R.B.P.,da,Silva,and,Paulo 摘要: Eukaryote genomes are endowed with varying quantities of repeated DNA ...
Randomly broken fragment PCR with 59 end-directed adaptor for genome walking Wentao Xu1,2*, Ying Shang1*, Pengyu Zhu1, Zhifang Zhai1, Jing He2, Kunlun Huang1,2 & Yunbo Luo1,2 1Laboratory of Food Safety, College of Food Science and Nutritional Engineering, China Agricultural University, ...
B., Dubé, A. K., Landry, C. R. Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells. J. Vis. Exp. (97), e52255, doi:10.3791/52255 (2015). Download Citation Reprints and Permissions JoVE 政策 •...