Here, we performed a genome-wide barcoded-CRISPRa screen to identify novel host factors that enable SARS-CoV-2 infection. Beyond known host proteins, i.e., ACE2, TMPRSS2, and NRP1, we identified multiple host components,among which LDLRAD3, TMEM30A, and CLEC4G were confirmed as ...
genome-wide CRISPR interference and CRISPR activation screens in human neurons. We uncover pathways controlling neuronal response to chronic oxidative stress, which is implicated in neurodegenerative diseases. Unexpectedly, knockdown of the lysosomal protein prosaposin strongly sensitizes neurons, but not ...
The CRISPR activation system was used to upregulate the PTPRD gene directly in K562 cells (Fig. 1i), which mimics the effects of NCRE de_novo_1 removal. A similar growth disadvantage was observed in cells with the direct upregulation of the PTPRD gene (Fig. 1j). The PTPRD gene knockout...
To date, only a small-scale RNA interference screen has been performed in vivo on mouse T cells (Chen et al., 2014). However, recently developed CRISPR technology has the advantages of higher specificity and greater flexibility, allowing knockout, repression, and activation (Adli 2018). ...
Genes involved in regulating Hippo pathway activation were also identified as CRISPR screen resistance hits in both lung and head and neck cancer cell lines, suggesting that this may be an important mechanism of resistance among other tumour types and not limited to the colon.Here we show that ...
Genome wide CRISPR screen in SH-SY5Y neuroblastoma cell line To perform a genome-wide CRISPR screen in search of modulators of tau protein levels, cells from the pooled cell line (PC1) were infected with a pooled lentiviral gRNA library encoding 90,000 individual guides targeting 18,360 human...
Fig. 2: Genome-wide CRISPR/Cas9 screen identifies host factors using Sdel virus as model. aSchematic of the screening process. A549 cells expressing the human ACE2 were used to generate the CRISPR sgRNA knockout cell library. The library was infected with Sdel strain of SARS-CoV-2, and ...
Aggressive myeloid leukemias such as blast crisis chronic myeloid leukemia and acute myeloid leukemia remain highly lethal. Here we report a genome-wide in vivo CRISPR screen to identify new dependencies in this disease. Among these, RNA-binding proteins
Altogether, our in vivo genome-wide CRISPR screen displayed adequate library coverage, provided high-confidence level gene hit datasets from both positive and negative selections and further defined potential cancer vulnerabilities and tumor suppressor gene hypersensitivity in TNBC. Activation of the mTOR ...
To identify genes whose deletion augments efficacy of the G12Cis adagrasib (MRTX-849) or adagrasib plus TNO155 (SHP2i), we performed genome-wide CRISPR/Cas9 screens on KRAS/STK11-mutant NSCLC lines. Recurrent, potentially targetable, synthetic lethal (SL) genes were identified, including ...