We perform genome-wide CRISPR/Cas9 deletion screening in normoxia-glucose, hypoxia-glucose and normoxia-galactose conditions, and identify both unique and overlapping genes whose loss influences tumour cell viability under these different metabolic conditions. We discover that loss of certain oxidative ...
Human leukocyte antigen (HLA)-independent, T cell–mediated targeting of cancer cells would allow immune destruction of malignancies in all individuals. Here, we use genome-wide CRISPR–Cas9 screening to establish that a T cell receptor (TCR) recognized and killed most human cancer types via the...
& Wei, W. High-throughput screens in mammalian cells using the CRISPR-Cas9 system. FEBS J. 282, 2089–2096 (2015). Article CAS Google Scholar Zhu, S., Zhou, Y. & Wei, W. Genome-wide CRISPR/Cas9 screening for high-throughput functional genomics in human cells. Methods Mol. Biol. ...
the development of drug resistance is common. By using genome-wide CRISPR/Cas9 library screening, we identify phosphoglycerate dehydrogenase (PHGDH), the first committed enzyme in the serine synthesis pathway (SSP), as a critical driver for Sorafenib resistance. Sorafenib treatment activates SSP by in...
Using genome-wide Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas)9 (CRISPR/Cas9) screening, we identified mitochondrial activity as the driver of panobinostat resistance in ALL. Mechanistically, ectopic SIRT1 expression activated mitochondrial activity and sensitized...
Functional genomic screening using the CRISPR-Cas9 system has emerged as a powerful approach to associate genetic perturbations with a distinct phenotype in cancer cells [57]. In particular, genome-wide loss-of-function screens in established cancer cell models allow for the identification of genetic...
www.nature.com/scientificreports OPEN A genome‑wide CRISPR/Cas9 screen to identify phagocytosis modulators in monocytic THP‑1 cells Benjamin Lindner1*, Eva Martin1, Monika Steininger2, Aleksandra Bundalo2, Martin Lenter1, Johannes Zuber2,3 & Michael Schuler1 Phagocytosis...
Genome-wide CRISPR-Cas9 KO library screening has enabled us to identify the important roles of PTPMT1 in hypoxic adaptation in cancer. PTPMT1 is an important enzyme for the synthesis of CL, an important component of the MIM. Proper CL synthesis is crucial to assembly of ETC complexes, which...
In an attempt to discover potential targets in the TNF-α signaling pathway to modulate moderate inflammation and apoptosis during the development of sepsis, we performed a pooled genome-wide CRISPR/Cas9 knockout screen in human umbilical vein endothelial cells (HUVECs). Tumor necrosis factor ...
Single-cell CRISPR screening is a powerful method for biological discovery. Such screens genetically perturb many genes in parallel and use single-cell RNA sequencing to assess the transcriptomic effects. Now, an in vivo protocol facilitates screens in mouse brain, using adeno-associated virus with ...