CRISPR-/Cas9Mammalian cellsgRNAForward genetic screens are a powerful and unbiased approach for uncovering the genetic basis behind a specific phenotype. Genome-wide mutagenesis followed by phenotypic screening represents the ultimate manifestation of this method, directly linking biological phenomena to its...
Genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) screening is a simple screening method for locating loci under specific conditions, and it has been utilized in tumor drug resistance research for finding potential ...
Human leukocyte antigen (HLA)-independent, T cell–mediated targeting of cancer cells would allow immune destruction of malignancies in all individuals. Here, we use genome-wide CRISPR–Cas9 screening to establish that a T cell receptor (TCR) recognized
the development of drug resistance is common. By using genome-wide CRISPR/Cas9 library screening, we identify phosphoglycerate dehydrogenase (PHGDH), the first committed enzyme in the serine synthesis pathway (SSP), as a critical driver for Sorafenib resistance. Sorafenib treatment activates SSP by in...
Here, we identified calcium-regulated heat-stable protein 1 (CARHSP1) as a critical driver for radioresistance utilizing genome-wide CRISPR activation screening. This is a protein with a cold-shock domain (CSD)-containing that is highly similar to cold-shock proteins. CARHSP1 mRNA level was up...
Genome engineering using CRISPR/Cas9 technology enables simple, efficient and precise genomic modifications in human cells. Conventional immortalized cell lines can be easily edited or screened using genome-wide libraries with lentiviral transduction. Ho
1c). The robust ranking algorithm model-based analysis of genome-wide CRISPR–Cas9 knockout (MAGeCK) was used to identify potential NCREs that affect cell growth on the basis of the screening data43. There were 346 UCEs and other potential NCREs depleted in the cell population that further ...
& Wei, W. High-throughput screens in mammalian cells using the CRISPR-Cas9 system. FEBS J. 282, 2089–2096 (2015). Article CAS Google Scholar Zhu, S., Zhou, Y. & Wei, W. Genome-wide CRISPR/Cas9 screening for high-throughput functional genomics in human cells. Methods Mol. Biol. ...
Functional genomic screening using the CRISPR-Cas9 system has emerged as a powerful approach to associate genetic perturbations with a distinct phenotype in cancer cells [57]. In particular, genome-wide loss-of-function screens in established cancer cell models allow for the identification of genetic...
control optimal expression conditions for objective production [46]. Unlike overexpression based on cDNA transduction, CRISPRa mainly promotes the expression of target genes by activating the promoter region of target genes, making it more suitable for a wide range of gene overexpression screening (...