Genome sequencing is backed by automated DNA sequencing methods and computer software to assemble the enormous sequence data. It can be divided into four stages: (1) preparation of clones comprising the entire genome of an organism; (2) collection of DNA sequences of clones; (3) generation of...
The first sequencing methods (the Maxam-Gilbert and Sanger methods), developed in the 1970s, weredeployedto reveal the nucleic acidcompositionof individual genes and the relatively small genomes of certain viruses. The sequencing of larger genomes remained out of reach conceptually, because of high ...
h2estimates from all the variants from WGS TOPMed cohort against array-genotypes captured in MGB Biobank to understand the differences contributed by these two sequencing methods. As expected, the h2estimates from array-genotypes were reduced corresponding to missing heritability from the lower MAF bins...
Marine Fungi are potent secondary metabolite producers. However, limited genetic information are available their biosynthetic gene clusters (BGCs) and their biotechnological applications. To overcome this lack of information, herein, we used next-generation sequencing methods for genome sequencing of two ma...
2022年5月30日,贝勒医学院人类分子遗传系吴涛课题组在Genome Biology发表了题为NT-seq: A chemical-based sequencing method for genomic methylome profiling的研究论文。该研究报道了一种基于化学转化的多种DNA甲基化测序的新方法(NT-seq...
Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression. Nature Genet. 2, 173–179 (1992). Article CAS PubMed Google Scholar Hillier, L. D. et al. Generation and analysis of 280,000 human expressed sequence tags. Genome Res. 6, 807–828 (1996...
Systematic comparative analysis of single-nucleotide variant detection methods from single-cell RNA sequencing data 长按识别二维码 阅读论文 Genome Biology Benchmarking Studies 点击“阅读原文”浏览专辑全部文章 Benchmarking Studies BMC是施普林格∙自...
A comparison of automatic cell identification methods for single-cell RNA sequencing data Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses Comprehensive evaluation of structural variation detection algorithms for who...
Over the past decade, long-read, single-molecule DNA sequencing technologies have emerged as powerful players in genomics. With the ability to generate reads tens to thousands of kilobases in length with an accuracy approaching that of short-read sequencing technologies, these platforms have proven ...
Restriction-enzyme (RE)-based next-generation sequencing methods have revolutionized marker-assisted genetic studies; however, the use of REs has limited their widespread adoption, especially in field samples with low-quality DNA and/or small quantities of DNA. Here, we developed a PCR-based procedu...