PolyploidCropimprovementSequence-specific nucleases (SSN) that generate double-stranded DNA breaks (DSBs) in genes of interest are the key to site-specific genome editing in plants.Genome editing has developed into one method of reducing undesirable traits in crops by the induction of knockout ...
, genome editing technology can not only mutate a single gene without affecting other members in a gene family, but also edit multiple genes of interest simultaneously through a single transformation, providing a perfect solution for facilitating the research of soybean and other polyploid crops....
A. rhizogenes mediated transient assay was performed in protoplasts of Silene latifolia by employing CRISPR/Cas9 tool for demonstrating mutations at T1 generation (Hudzieczek et al., 2019). The cleavage activity of Cas9 was demonstrated to edit the host gene and also remove the T-DNA region in...
In addition to the already-mentioned problems, in polyploid crops the GE protocols should enable simultaneous targeting of multiple alleles, which could be an obstacle for GE of wheat and other crops with complex genomes. Thus, it is crucial to determine the efficiency of the sgRNAs selected ...
CRISPR/Cas9 is a valuable tool for both basic and applied research that has been widely applied to different plant species. Nonetheless, a systematical assessment of the efficiency of this method is not available for the allotetraploid Brassica napus—an
(Fig.2h). Collective analysis of the editing data further confirmed that LrCas9 can edit all 5’-NGAAA-3’ PAM sites and the editing efficiencies at different 5’-NGAAA-3’ PAM groups showed no significant difference (Fig.2i). By analyzing the genome editing outcomes among all these ...
(2018). Application of CRISPR/Cas9 genome editing technology for the improvement of crops cultivated in tropical climates: Recent progress, prospects, and challenges. Frontiers in Plant Science, 9, 617. https://doi.org/10.3389/fpls.2018.00617. Article PubMed PubMed Central Google Scholar ...
such as which genes are highly expressed during sugar production, or which genes are important for disease resistance. This study found that the genes responsible for resistance to brown rust, a fungal pathogen that previously caused millions of dollars of damage to sugarcane crops, are found i...
Genome editing of polyploid crops: prospects, achievements and bottlenecks 2021, Transgenic Research CRISPR/Cas9-mediated editing of DMR6 orthologue in banana (Musa spp.) confers enhanced resistance to bacterial disease 2021, Plant Biotechnology Journal ...
In autopolyploid meiosis, there are many distinct features with the inheritance of diploid and allopolyploid, such as multivalent pairing and preferential pairing of chromosomes, and double reduction (DR), which have been long-standing research interest in tetraploid potato and other polyploid crops (...