K., 2015. Genome editing in human cells using CRISPR/Cas nucleases. Curr. Protoc. Mol. Biol. 112 : 31.3.1–31.3.18.Wyvekens, N., Tsai, S.Q., and Joung, J.K. (2015). Genome editing in human cells us- ing CRISPR/Cas nucleases. Curr. Protoc. Mol. Biol. 112, 31.3.1-...
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is a versatile and convenient genome-editing tool with prospects in gene therapy. This technique is based on customized site-specific nucleases with programmable guiding RNAs that cleave and intr...
Genome Editing with CRISPR-Cas9 (Chinese Translation)是【中文字幕】Prof Jennifer Doudna- CRISPR Cas9, Genome Editing and the Future的第2集视频,该合集共计2集,视频收藏或关注UP主,及时了解更多相关视频内容。
张锋博士在科罗拉多大学博尔德分校关于CRISPR-Cas进展的讲座-Genome Editing Using CRISPR-Cas Systems 115 1 6:27 App Mechanisms and secrets of Alzheimer's disease exploring the brain 4798 14 1:36:53 App 【公开课录屏】【微观生物】【进阶课】CRISPR/Cas系统及其应用 2020.5.6 1406 1 1:06:28 App 【...
TRANSFECTION TIPS VIDEO: CRISPR/Cas9 Transfection This video covers the basic mechanism of CRISPR/Cas9 genome editing and introduces the key points affecting researchers’ decisions about whether to deliver Cas9 and guide RNA in plasmid, RNA or RNP format. ...
根据第一段中“Using CRISPR genome (基因组) editing on a few common crops, a team of plant and soil scientists seeks to greatly increase and speed up carbon storage to help fight climate change.(一个由植物和土壤科学家组成的团队试图通过对几种常见作物进行CRISPR基因组编辑,大幅增加和加速碳储存,...
2. Cas9: an RNA-guided nuclease for genome editing 详细介绍Cas9,并且介绍sgRNA。 3. Comparison with other genome editing technologies 简单的对比一下不同的基因编辑工具的优缺点,突出CRISPR-Cas9的优点 4. Limitations of the Cas9 system 简述CRISPR-Cas9的缺点 ...
RNA-programmed genome editing in human cells. Elife 2, e00471 (2013). PubMed PubMed Central Google Scholar Shalem, O., Sanjana, N. E. & Zhang, F. High-throughput functional genomics using CRISPR-Cas9. Nat. Rev. Genet. 16, 299–311 (2015). CAS PubMed PubMed Central Google Scholar...
Using CRISPR genome (基因组) editing on a few common crops, a team of plant and soil scientists seeks to greatly increase and speed up carbon storage to help fight climate change. To prevent dangerous levels of global warming, scientists say it won't be enough to just stop burning fossil...
Are you genome editing with CRISPR-Cas9? Consider the Alt-R CRISPR-Cas9 kit—a customizable, end-to-end Cas9-CRISPR System offering best in class performance.