To this end, we compared the genome editing specificities of the PAM-based and conventional methods on an oncogenic single-base mutation in the endothelial growth factor receptor (EGFR). The results indicated that the PAM-based method provided a significantly increased genome editing specificity for ...
Over the last few years, the exuberant development of genome editing has revolutionized research on the human genome, which has enabled investigators to better understand the contribution of a single-gene product to a disease in an organism. In the 1970s, the development of genetic engineering (m...
In many areas of oncology, we lack sensitive tools to track low-burden disease. Although cell-free DNA (cfDNA) shows promise in detecting cancer mutations, we found that the combination of low tumor fraction (TF) and limited number of DNA fragments restr
Liquid biopsy based on cell-free DNA (cfDNA) analysis holds significant promise as a minimally invasive approach for the diagnosis, genotyping, and monitoring of solid malignancies. Human tumors release cfDNA in the bloodstream through a combination of e
There are known disparities in incidence and outcomes of colorectal cancer (CRC) by race and ethnicity. Some of these disparities may be mediated by molecular changes in tumors that occur at different rates across populations. Genetic ancestry is a measu
(Fig.2d). Instead, these were prevalent within individual patients suggesting that specific tumor genotypes could be linked to unique repertoires of tumor cell programs and that larger xenograft cohorts will likely reveal additional insights. In support of this, previous work establishing that EGFR ...
The VEP also provides an indication of the effect of the amino acid change using protein biophysical properties. These data can improve interpretation of protein variants with no associated phenotype or disease data by predicting how deleterious a given mutation may be on the functional status of ...
The nuclear lamina regulates germline stem cell niche organization via modulation of EGFR signaling Cell Stem Cell, 13 (2013), pp. 73-86 View in ScopusGoogle Scholar Chen et al., 2014 H. Chen, X. Zheng, Y. Zheng Age-associated loss of lamin-B leads to systemic inflammation and gut hype...
Triple negative breast cancer (TNBC) is defined as lacking the expressions of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). TNBC patients exhibit relatively poor clinical outcomes due to lack of
myeloid leukemia according to AACR GENIE. Amplification ofCBLCreportedly causes competition with CBL and inhibits EGFR degradation, thereby increasing the stability of activated EGFR and enhancing cell proliferation21. The suppressive effect ofSLC34A2on tumorigenesis and progression via down-regulation of ...