The present invention relates to the field of analysis of the three-dimensional structure of the genome, i.e., for genome architecture mapping (GAM). The invention provides a method of determining spatial proximity of a plurality of nucleic acid loci in a compartment such as the cell nucleus,...
b, GAM has few of the limitations that affect current genome-wide methods for mapping genome architecture. c, In 3C-based methods, the presence of multiple loci in a single interaction may dilute the measured ligation frequency between any two member loci. In GAM, the measured interaction is ...
A technique called genome architecture mapping (GAM) involves sequencing DNA from a large number of thin nuclear cryosections to develop a map of genome organization without the limitations of existing 3C-based methods. Robert A. Beagrie Antonio Scialdone ...
Methods for Mapping Three-Dimensional Genome Architecture Daniel J.McKay, ...Jill M.Dowen, inMethods, 2020 2Features of genome organization In order for the genome to function properly, packaging of genetic material into the nucleus must allow for dynamic DNA-templated processes like transcription,...
【7】 Mumbach MR, Rubin AJ, Flynn RA, Dai C, Khavari PA, Greenleaf WJ, Chang HY:HiChIP: efficient and sensitive analysis of protein-directed genome architecture.Nat Methods2016,13:919-922. 【8】 Zhou X, Maricque B...
Genome architecture describes how genes and other features are arranged in genomes. These arrangements reflect the evolutionary pressures on genomes and underlie biological processes such as chromosomal segregation and the regulation of gene expression.
Association mapping is a powerful approach for dissecting the genetic architecture of complex quantitative traits using high-density SNP markers in maize. Here, we expanded our association panel size from 368 to 513 inbred lines with 0.5 million high quality SNPs using a two-step data-imputation m...
Lineage-specific genome architecture links enhancers and non-coding disease variants to target gene promoters. Cell. 2016;167:1369–1384.e19. Article CAS PubMed PubMed Central Google Scholar Ernst J, Kellis M. ChromHMM: automating chromatin-state discovery and characterization. Nat Methods. 2012;...
Methods for mapping 3D chromosome architecture. Nat. Rev. Genet. 21, 207–226 (2020). CAS PubMed Google Scholar McCord, R. P., Kaplan, N. & Giorgetti, L. Chromosome conformation capture and beyond: toward an integrative view of chromosome structure and function. Mol. Cell 77, 688–708...
M. et al. The Xist lncRNA exploits three-dimensional genome architecture to spread across the X chromosome. Science 341, 1237973–1237973 (2013). Article Google Scholar Terranova, C. et al. An integrated platform for genome-wide mapping of chromatin states using high-throughput chip-sequencing...