本发明公开了一种芒属植物Genic-SSR标记的制备方法及应用,其步骤是:A,芒属植物转录组序列的获得:选取芒属植物的幼嫩叶片,分别提取总RNA,得到转录组Reads,去除载体RNA序列污染,拼接,获得芒属植物转录组序列;B,芒属植物ESR序列中SSR位点的鉴定:采用SSR检索软件对得到的非冗余Unigene-EST序列进行SSR位点查找,重复基序...
A subset of 30 SSR markers were randomly selected and validated, yielding 19 polymorphic markers with an average polymorphism information content value of 0.431. The development of genic-SSR markers in this study is indeed timely to provide useful markers for functional and population genetic studies...
本研究通过转录组测序技术,构建了四合木基因表达数据库;通过多组转录组比较,系统识别多态性genic-SSRs,并对其进行了数目,频率及在基因中的位置等特性分析,开发了分子标记;最后利用所开发genic-SSR标记开展该物种的种群遗传学研究,解析幼株与成株群体遗传多样性和种群动态,主要成果如下:(1)经测序,共获得343.2百万条...
【目的】对授粉时未做去雄处理的‘满天红’ב红香酥’组合进行杂种鉴定和遗传变异分析,排除非双亲杂种单株,为利用该组合进行连锁分析奠定基础。【方法】用9对从转录组数据开发的在双亲间表现多态性的genic-SSR引物和1对自交不亲和基因检测引物对‘满天红’ב红香酥’组合所有单株进行杂种鉴定、标记偏分离分析和...
The 32 polymorphic primer pairs were validated using an F2 mapping population; 18 primer pairs exhibited polymorphisms between the parents, and 14 genic-SSRs could be integrated into 9 main linkage groups. Conclusions 2,164 genic-SSR markers have been developed in sesame using transcriptome ...
We developed 550 validated genic-SSR markers in pigeonpea using deep transcriptome sequencing. From these, 20 highly polymorphic markers were used to evaluate the genetic relationship among species of the genus Cajanus. A comprehensive set of genic-SSR markers was developed as an important genomic re...
与'红香酥'聚到一起.另有79个后代,单独聚在一起.【结论】利用Genic-SSR分析成功地对'满天红'×'红香酥'组合梨杂种后代进行了鉴定,表明外来花粉污染是造成杂交群体中非双亲杂种污染的主要原因,产生自交后代污染的概率较低,聚类分析表明S-RNAse和部分SSR位点在该杂交群体中发生偏分离,半数以上的单株与父本'红香酥'...
The transcriptome sequencing data and millet-specific SSR markers developed in this study establish an excellent resource for gene discovery and may improve the development of breeding programs in proso millet in the future. 展开 关键词: de novo transcriptome genetic diversity genic SSR germplasm ...
[translate] aUsing primers batch design software were developed 1569 pairs of genic-SSR primers 使用底漆批设计软件被开发了1569个对遗传因子的SSR底漆[translate]
Pollegioni P; Woeste K; Mugnozza GS;.Retrospective identification of hybrid ogenic walnut plants by SSR fingerprinting and parentage analysis.Molecular Breeding.2009.321-335Retrospective identification of hybridogenic walnut plants by SSR fingerprinting and parentage analysis[J] . Paola Pollegioni,Keith...