CRISPRi knockdown of essential genes combined with TnSeq of non-essential genes yields a high-throughput method for bacterial genetic interaction mapping. Bimal Jana Xue Liu Tim van Opijnen ResearchOpen Access19 Jul 2024Nature Microbiology
When using GRETTA, please cite the manuscript describing GRETTA: Yuka Takemon, Marco A Marra, GRETTA: an R package for mapping in silico genetic interaction and essentiality networks, Bioinformatics, Volume 39, Issue 6, June 2023, btad381,https://doi.org/10.1093/bioinformatics/btad381 ...
wherein disruption of an interaction between a protein of interest and its binding partner protein is assayed for a library of mutations of said protein of interest, and maintenance of an interaction between the protein of interest and another binding partner is assayed simultaneously in a single ...
Our approach involves five main components, each of which is described briefly below (Fig.1cand Supplementary Fig.1; see Methods): (I) Data processing; (II) construction of a SNP–SNP interaction network; (III) binarization of the SNP–SNP network; (IV) scoring of BPMs and WPMs for enri...
wherein disruption of an interaction between a protein of interest and its binding partner protein is assayed for a library of mutations of said protein of interest, and maintenance of an interaction between the protein of interest and another binding partner is assayed simultaneously in a single ...
Body composition and gene expression QTL mapping in mice reveals imprinting and interaction effects Previously, we found that Myostatin genotype, reciprocal cross, and sex interacted with numerous chromosomal regions to affect growth traits. Results Here, ... Y Cheng,S Rachagani,A Cánovas,... - ...
heritable. Analogous to the traditional genetic study, most researchers treat the expression of one gene as a quantitative trait and map it to expression quantitative trait loci (eQTL). This is 1D-trait mapping. 1D-trait mapping ignores the trait-trait interaction completely, which is a major ...
第一阶段:连锁分析linkage mapping找疾病相关的coding mutation 背景:此时我们已经知道性状表型是由基因控制,且认为基因功能受编码区影响(非编码区的功能还不清楚),也已经掌握了连锁分析来定位疾病相关的基因,主要是从家系分析开始。 局限性:rare coding variants in RET appear to play a less prominent role in spor...
Tonikian R, Roberts T, Sdicu AM, Shapiro J, Sheikh B, Suter B, Wong SL, Zhang LV, Zhu H, Burd CG, Munro S, Sander C, Rine J, Greenblatt J, Peter M, Bretscher A, Bell G, Roth FP, Brown GW, Andrews B, Bussey H, Boone C: Global mapping of the yeast genetic interaction ...
Jerry DR, Kvingedal R, Lind CE, Evans BS, Taylor JJU, Safari AE: Donor-oyster derived heritability estimates and the effect of genotype x environment interaction on the production of pearl quality traits in the silver-lip pearl oyster, Pinctada maxima. Aquaculture. 2012, 338–341: 66-71. ...