RNA was extracted from cells using Trizol reagent (Gibco-BRL, Rockville, Md.) and isolated as specified by the manufacturer. The RNA was DNAse-treated (DNase I-RNase-Free, Ambion) to remove any contaminating DNA; 200 ng of total RNA was reverse-transcribed with oligo dT primers using ...
38,39,40,41,42]. Indeed, in reciprocal co-immunoprecipitation assays, AMOTp130 interacted with WWC1, WWC2, or WWC3 (Fig.2A-Cand S2A–C). This interaction was abolished when either WW domains in WWC1 were mutated or deleted, or when the third PPxY motif of AMOTp130 was mutated, in...
Preparation of the virus RSV (long Strain, ATCC VR-26) obtained from the American Type Culture Collection (ATCC, Rockville, MD) was propagated in Hep-2 cells in DMEM (Invitrogen Ltd., Paisley, UK) with 2% fetal calf serum (DMEM-2; Invitrogen Ltd.), as previously described ...
For recombinant protein expression, mCAP2, mTmod1, or mCFL2 with an N-terminal 6xHistidine-tag was cloned into pReceiver-B01 (GeneCopoeia, Rockville, MD). Protein expression and purification Globular actin (G-actin) was purified from actin acetone powder69 obtained from rabbit skeletal muscle ...
response and immune cell infiltration. The sections were also immunostained with antibodies against NLRP3 (catalog no. 19771–1-AP; dilution, 1:500; negative control). The levels of CD45 and NLRP3 expression were measured using Image-Pro Plus (version 6.0; Media Cybernetics, Rockville, MD, ...
Lymphoproliferative diseases include many different disease entities with distinct cells of origin, pathologies, risk factor profiles, and prognoses. Although lymphomas are grouped traditionally into non-Hodgkin and Hodgkin lymphomas, the WHO classificat
Media were analyzed for Aβ42 by MSD Multiplex ELISA (Meso Scale Discovery, Rockville, MD, USA). Three independent experiments were performed, with three sample replicates each. Statistical analyses For the statistical analyses examining the putative effects on continuous response variables, we used ...
Adhesion and aggregation of platelets on fibrin or fibronectin-fibrin under shear conditions were studied using a parallel plate flow chamber (Glycotech, Rockville, MD) in which a silicone rubber gasket with a thickness of 0.127 mm and a flow path width of 2.5 mm were placed on a 3.5-cm dia...
A full-length human JDP2 cDNA was obtained from Origene (Rockville, MD, USA). NIH3T3 cells grown in 96-well plates were transfected with (i) either 100 ng Trp53-Luc (black lines) or mutTrp53-Luc (grey lines), (ii) 20 ng pRL-SV40 (an internal control reporter; Promega) and (iii...
We used H9c2 cells (ATCC CLR-1446; Rockville, MD, USA) derived from rat embryonic myoblasts as anin vitromodel of cardiomyocytes biology, since they show similar ions currents responses to primary adult and neonatal cardiomyocytes. The cells were plated onto collagen-coated culture dishes and cult...