A system used to determine the existence of direct interactions between proteins. It involves the use of plasmids that encode two hybrid proteins; one of them is fused to the GAL4 DNA-binding domain and the other one is fused to the GAL4 activation domain. The two proteins are expressed to...
6). Next, we set out to determine the contribution to biofilm formation for the individual genes within the APEEc BGC. Only the ΔapeE (ACP) and ΔapeN (LolB-like protein) strains showed a significant increase in biofilm formation when compared to APE− (Fig. S4). Since APEEc ...
These may be used to determine whether variants similar to the regulatory variant under study are known for a disease phenotype (C2.4). Current Data for Published Variants: Variant Frequencies and In Silico Impact Predictions Two key bioinformatic resources include variant frequencies in background ...
Adominantallele is avariation of a genethat will produce a certain phenotype, even in the presence of other alleles. Only one allele of the dominant variety needs to be present to show in the individual as a characteristic. An example of this would be brown eye color in humans, which is...
1A). It can be intuitively shown that the correlation indices of the mapped genes in different groups were different, but the expression patterns within the groups were similar. To determine whether the samples were clustered based on MMVD, we performed PCA on the quantitative genes of the ...
is used to predict the possible genotype and phenotype of offspring. It operates on Mendelean principles. As a reference, the genotype is the genetic makeup of an individual and thephenotypeis the expression of the genetic makeup. In other words, the phenotype is what the organism looks like...
(Fig.8). We calculated the log2-fold change (FC) values between the RNA-seq and RT-qPCR datasets to determine the correlation between them and analyzed the Pearson correlation coefficient. The results indicated that the RNA-seq and RT-qPCR datasets had a positive correlation coefficient (R2...
Comparisons were done using Wald’s test to determine the log2-fold change. To overcome the inconsistency of results of different studies and stabilize the genes’ expression ratios, batch effect correction by using the Empirical Bayes method was performed19. This correction enables direct comparisons...
melanogasterhave shown that disrupting ribosome function can result in an array of fascinating dominant phenotypes [1,2]. Despite this, there has so far been no comprehensive inventory of genes encoding ribosome components in this organism, nor any systematic effort to determine their mutant ...
In cells, a gene is a portion of DNA that contains both "coding" sequences that determine what the gene does, and "non-coding" sequences that determine when the gene is active (expressed). When a gene is active, the coding and non-coding sequences are copied in a process called trans...