If the enzyme is composed of multiple subunits, an individual heterozygous for the genes of some or all of the subunits will be capable of assembling a greater variety of types. Multiple genetic loci The organism may produce one protein with a given enzyme function in one tissue, and a ...
fast, and reproducible method to confirm large-scale gene expression data. The use of stable reference genes for the normalization of RT-qPCR assays is recognized worldwide. No systematic study for selecting appropriate reference genes for usage in RT-qPCR experiments comparing gene expression...
The cDNAs for the second, third, and fourth cDNA libraries were synthesized using total RNAs derived from the aerial part of the chrysanthemum with a Mint-2 cDNA synthesis kit (evrogen; http://evrogen.com/) according to the manufacturer’s instructions. The first strand cDNAs were synthesized ...
Blackgram [Vigna mungo (L.) Hepper] (2n = 2x = 22), an important Asiatic legume crop, is a major source of dietary protein for the predominantly vegetarian population. Here we construct a draft genome sequence of blackgram, for the first time
Subsequent exposure to sucrose can eliminate cells that contain a fusion of the suicide plasmid and the native-cryptic plasmid. Notably, segregation conditions for engineered native-cryptic plasmids in cyanobacteria were optimized previously (Armshaw et al., 2015), suggesting that glucose-free media ...
antarctica was found to contain many genes for peroxisomal β-oxidation [42]. The enzymes responsible for MEL biosynthesis are compartmentalized to enable production of differently acylated MELs, and to allow for simultaneous assembly of different glycolipids in a single cell requiring the same ...
The probiotic treatment reduced the presence of some genera which contain potential pathogens (e.g. Mycobacterium) and enhanced the abundance of reads assigned to the class Bacilli. Reads from the Lactobacillus genera were significantly elevated by the probiotic lar- vae and in addition the probiotic...
Different Ras-like GTPases contribute to these activities by acting as key regulators in assembling specific protein complexes at different donor and target membranes [21–23]. In S. cerevisiae, GYP8 encodes a GTPase-activating protein for Ypt/Rab GTPases, which plays an essential function in ...
manufacturer’s instructions and employed partial runs with a manifold to separate samples. To assemble our transcriptomes, we used GS De novo Assembler v2.3 (“Newbler”; 454 Life Sciences/Roche Branford, CT USA) set to default threshold options, and using the –vt option to remove adapters. ...
After assembling our list of genes for LIT 1.0, we used functionally characterized exemplars of each of these genes (i.e.those from model systems such as fly or mouse; see Additional file1: Table S1) and the blastp algorithm to search the predicted protein databases associated with 29 fully...