Interference matrix (IM) has been widely used in frequency planning/optimization of cellular systems because it describes the interaction between any two cells. IM is generated from the source data gathered from the cellular system, either mobile measurement reports (MMRs) or drive test (DT) recor...
Traditional methods for the generation of DNA damage are not well suited for the observation of spatiotemporal aspects of damaged chromosomal loci. We describe a protocol for the derivation of a cellular system to induce and to visualize chromosome damage at specific sites of the mammalian genome ...
Cellular therapy updates in B-cell lymphoma: the state of the CAR-T. Cancers (Basel) 13, 5181 (2021). Albinger, N., Hartmann, J. & Ullrich, E. Current status and perspective of CAR-T and CAR-NK cell therapy trials in Germany. Gene Ther. 28, 513–527 (2021). Article CAS Pub...
Evaluation of cellular uptake To investigate the cellular uptake, 4T1 cells were seeded onto 35 mm glass-bottom dishes at a density of 1 × 105 cells incubated with NPs and N-NPs (10 μg/mL) at 37 °C for 4 h, respectively. Next, the cells were fixed by 4% paraformal...
Intestine-Specific Ablation of Mouse atonal homolog 1 (Math1) Reveals a Role in Cellular Homeostasis Math1 (Atoh1) is a basic helix-loop-helix transcription factor important for intestinal secretory cell differentiation. We hypothesized that Math1 is impor... NF Shroyer,MA Helmrath,YC Wang,.....
Direct evidence that cells of the hair follicle bulge can be induced to form new hair follicles has been presented (Morris et al, 2004). Although neofolliculogenesis is not generally believed to occur normally in the adult state, new follicle formation can be induced experimentally by cellular ...
This first-generation system for the construction of human artificial chromosomes should be suitable for dissecting the sequence requirements of human centromeres, as well as developing constructs useful for therapeutic applications. 展开 关键词: Humans Tumor Cells, Cultured Chromosomes, Human Telomere ...
We generated a human cellular atrial fibrillation model starting from a patient carrying KCNA5 p.D322H mutation, already described as a gain of function mutation (Christophersen et al., 2013). We believe that the analysis of iPSC-derived mutated cardiomyocytes will contribute to elucidate the mole...
For cellular activation assays, DN32.D3 hybridoma cells are typically incubated with lipid-loaded CD1d. To provide a more physiological stimulation, particularly for lipid antigens that require processing, a cellular CD1d-lipid presentation system would be advantageous. To this end, we introduced human...
Ex vivo perfusion of rat and porcine organs and decellularization of the vascular tree. (a) Schematic representation of the hypothermic perfusion system used to decellularize rat and porcine organs. The circuit was driven by a peristaltic pump (Masterflex, Cole-Parmer) and controlled using a pr...