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Cut 1-mm fragments of tissue were then placed on glass slides with spacers (Grace Bio-Labs, 654002), cleared using FocusClear (CelExplorer, FC-101) for 30 min, and VectaShield mounting medium (Vector Laboratories) and a coverslip were applied. Imaging was performed on tissues from at least...
Supplementary Fig. 1E). Purified cells were grown in RPMI-1640 supplemented with L-glutamine and NaHCO3(Sigma-Aldrich, Cat. No. R8758) and 10% (v/v) FBS (fetal bovine serum) (Thermofisher Scientific, Cat. No. 16000069), as well as penicillin-streptomycin...
(C) Cell type differentiation at 10-nM DIF-1. AX3 cells co-transformed withpspA,ecmAO, orecmBcell type specific reporters were incubated with 10-nM DIF-1 and the number of expressing cells determined by FACS (seeSTAR Methods). (D) Cell type differentiation is cell-cycle-dependent. 448 ...
Image used with permission by copyright holder Phishing and malware protection in Docs, Sheets, and Slides top out the list of announced features for Workspace products. With all of these being powered by Artificial Intelligence, Google says these new features make their products more helpful and ...
For the preparation of GUVs, electroformation method was used, which yields unilamellar vesicles with diameter ranging from 5 to 100 μm (Kahya et al., 2005). The perfusion chamber used for vesicle preparation was equipped with two microscope slides, each coated with indium-tin oxide (ITO),...
A coverslip was placed into the slides with a medium containing 4,6-diamidino-2-phenylindole (DAPI) and scanned using a Zeiss AxioScan Z.1 (Zeiss) at 10X magnification. The coverslips were removed after 30 min soaking in washing buffer. Stripping of the antibodies was performed in a buffer...