(19 upland rice cultivars from the North, 99 lowland rice cultivars from the Northeast, 45 floating rice cultivars from the Northeast and 40 cultivars from the South), 21 recommended rice varieties and two susceptible varieties 'KDML105' and 'Nipponbare' by using gene-specific markers for the ...
TIP: The PCR protocol presented here includes incorporation of radioactivity into the PCR products. Radioactivity is necessary for visualization on a denaturing acrylamide gel for low abundant transcripts or when PCR product patterns are complicated (i.e., polymorphic markers for LOH). The amount of ...
5.Identification of Genetic Markers Linked to the Cytoplasmic Male Sterility Genes and Sequencing of the Specific Fragment in Non-heading Chinese Cabbage不结球白菜胞质雄性不育基因分子标记的筛选及特异片段的分析 6.Cloning of Cassava SBEI Gene Fragment and Construction of its Storage Root Specific Antise...
RNA interference (RNAi) Gene Specific Silencing RNA interference (RNAi) is a phenomenon in which the introduction of double-stranded RNA (dsRNA) into a diverse range of organisms and cell types causes degradation of the complementary mRNA (Figure 1; step 1). In the cel...
Aii J, Nagano M, Woo SH, Campbell CG, Adachi T (1999) Development of the SCAR markers linked to the S h gene in buck-wheat. Fagopyrum 16:19–22 Google Scholar Archak S, Gaikwad AB, Gautam D, Rao EVVB, Swamy KRM, Karihaloo JL (2003) Comparative assessment of DNA fingerprinting ...
(Figs.1,2) using theQmatrix method in the STRUCTURE program. TheQvalues, which range from 0 to 1, indicate the probability with which a genotype can be assigned to a particular cluster. The higher this number, the more reliable the genotype assignment. All 970 of the DArT markers with ...
To validate our datasets, we confirmed the expected expression pattern of known markers necessary for the development of sex-specific features, which typically appear during the third larval stage41.pkd-2, for instance, is expressed in the cilia of three different types of male sensory neurons42...
e, Top, accessibility browser tracks for each lineage split by day, highlighting peaks linked to late lineage markers (on-target: Aox3; off-target: Col28a1 and Vegfd) showing lineage-specific changes in accessibility on day 3. The Aox3- and Vegfd-linked DERs overlap perfectly with an ...
The pre-selection of SNPs could be based on elevated population differentiationFst, extended linkage disequilibrium LD, or phenotypic association. However, the determination could also depend on the data property and study purposes. For instance, if the positive selection acting on disease markers is ...
Radioactivity is necessary for visualization on a denaturing acrylamide gel for low abundant transcripts or when PCR product patterns are complicated (i.e., polymorphic markers for LOH). The amount of radioactivity used in the above protocol often results in visible products in less than 2 hours ...