Bundled with gene synthesisVectorArk™ Cloning Bundled with gene synthesisSubcloning Bundled with gene synthesis Service NumberSC0002SC0002SC0002 AdvantagesExpression vectors – Choose from over 150 popular vectorslistedSave on cloning into your archived vectorsGet ready to use clones in any vector, ...
DNA synthesis is rapidly becoming a crucial part of research workflow and has transformed modern biology. De novo gene synthesis is quickly replacing the classical cloning and mutagenesis procedures and allows generation of nucleic acids where no template is available. In addition, when coupled with ...
Gene cloning and transformation A 1.2% agarose gel was used to electrophorese the PCR product, and the band believed to include the desired gene was removed. The GeneJET Gel Extraction Kit from Thermo Scientific (Waltham, Massachusetts, United States) was used for processing. A 1500 bp fragment...
Gene synthesis and cloning. 1,4-BDO biosynthetic pathway genes (ald, 4hbd, cat2, and sucD; sequences are listed in Supplementary Table 2) for construction of YSB20 were synthesized using assembly PCR with 80 nt oligos. Sense oligos and non-sense oligos were overlapped by 40 nt. Assembly...
Sex-specific traits and behaviors emerge during development by the acquisition of unique properties in the nervous system of each sex. However, the genetic events responsible for introducing these sex-specific features remain poorly understood. In this s
The reader is introduced to the basic process of heterologous gene cloning and the advantages and limitations of each production system. The issues of expression vector structure, gene expression, and secretion are addressed for each of the systems and their ability to perform posttranslational ...
DNA synthesis is an increasingly attractive approach to avoid the cloning procedure entirely. Modern technologies permit the synthesis of DNAs greater than 10,000 base pairs long, so if the desired sequence is known, a commercial company can synthesize the DNA fragment in a plasmid backbone. An ...
The ability to clone large DNA fragments has enabled the cloning of entire biosynthetic clusters, some of which are very large.80 By modifying these clones to include site-specific integration sites, it may be possible to transfer them into strains for heterologous expression. Use of such ...
GenEZ™ supports direct cloning only into selected vectors. If you need cloning into a custom vector or a modified version of a commercial vector, please useSC1017 Gene Synthesis and Subcloning Bundle Service If you need codon optimization or other sequence modifications, please use our otherGen...
The availability ofgene knockoutfor specific mouse genes and the positional cloning of human genes responsible for familial situs abnormalities will allow the complete delineation of the intracellular events that translate and maintain positional information to the developing organs.Brown Anderson 1999; Cimb...