13,14,17,19,21. Still, splicing regulators represent only a small fraction of genes with the highest rates of unproductive splicing (Supplementary Note2). Further, we observe that introns with weak splice sites (Supplementary Fig.4) and long introns (Fig.2a) have higher unproductive...
13,14,17,19,21. Still, splicing regulators represent only a small fraction of genes with the highest rates of unproductive splicing (Supplementary Note2). Further, we observe that introns with weak splice sites (Supplementary Fig.4) and long introns (Fig.2a) have higher unproductive...
Large genes require dual adeno-associated viral (AAV) vectors for in vivo delivery/expression, but current methods have limitations. Here the authors develop and functionally evaluate REVeRT, an efficient and flexible dual AAV vector technology based on reconstitution via mRNA trans-splicing. ...
RNA-binding proteins potentially regulate the alternative splicing of cell cycle-associated genes in proliferative diabetic retinopathy Ning Yang , Ningzhi Zhang & Lei Du Article 19 March 2024 | Open Access Uncovering the molecular mechanisms of russet skin formation in Niagara grapevine (Vitis vi...
c, Knockdown of tra in adult females affects the alternative splicing between dsxF and dsxM. d, Effect of double knockdown of both tra and dsx in adult females on dsx isoforms. P values were determined by two-tailed unpaired t-test (b–d). Data in bar plots are mean ± s.e.m ...
(ORF) from the initiation tostop codonsmay imply the gene; however, the enhancers, promoters, splicing signals, and othercis-acting elements, which are collectively often called “regulatory sequences,” are also considered to be a part of the gene. To conductmutagenesisand functional analyses ...
One of the trending fields in almost all areas of science and technology is artificial intelligence. Computational biology and artificial intelligence can
, 2004), rendering the contribution of trans-splicing to the overall corrective effect unclear if selection is employed. Regardless of these technical considerations, for this technology to be useful in clinical applications, more efficient trans-splicing is needed. Although no nonspecific trans-...
Splicing factors (SFs) act in dynamic macromolecular complexes to modulate RNA processing. To understand the complex role of SFs in cancer progression, we performed a systemic analysis of the co-regulation of SFs using primary tumor RNA sequencing data. Co-regulated SFs were associated with aggressi...
Genomic organization and splicing evolution of the doublesex gene, a Drosophila regulator of sexual differentiation, in the dengue and yellow fever mosquit... and of the encoded sex-specific proteins, which are transcriptional regulators of downstream terminal genes that influence sexual differentiation ...