A protein module of∼110 amino acids that mediates interaction with acetylated Lys and is often found in acetyltransferases and ATP-dependent chromatin remodelling factors. Isothermal titration calorimetry A technique to analyse intermolecular interactions by directly measuring the heat generated or absorbed...
Subsystems of metabolites were defined according to the Supplementary Data 1. Protein overexpression and purification TFs were purified from the E. coli ASKA strains46. Cells were grown in 200 mL TB medium containing 30 µg × mL−1 chloramphenicol at 37 °C. When cells reached ...
Therefore, it is possible that amino acid accumulation supports increased production of metabolites that are part of a defense under very saline conditions. The concentration of several metabolites such as fructose, glycerol, malate, maltose, mannose, galactinol, myo-inositol, putrescine, raffinose, ...
Uniprot,全称Universal Protein,整合了EBI (European Bioinformatics Institute)、SIB (The Swiss Institute of Bioinformatics) 和PIR (Protein Information Resource) 三大数据库资源,是目前世界上使用最广泛的蛋白质数据库。其中UniprotKB收录蛋白质信息,由Swiss-Prot和TrEMBL两个子库构成。Swiss-Prot是高质量手工注释的非...
Diseases and disease network ontologies represent pathways involved in pathological processes, largely derived from literature data linking network objects (genes, proteins, metabolites) to disease. Ontology enrichment showed that HK genes, indeed, were enriched in vital cellular functions such as oxidative...
Fisher's exact test can be performed to test the significance of over or/and under representation of a set of similarly functioning genes, proteins or metabolites. These elements are counted after categorizing them into four sets A, B, C, and D. High A and C and low B and D indicate ...
Species in the Solanaceae family are known for producing plethora of specialized metabolites. In addition to biosynthesis pathways, a full comprehension of secondary metabolism must also take into account the transport and subcellular compartmentalization of substances. Here, we examined the MATE (Multidrug...
The concentrations of metabolites were measured using enzymatic assays or HPLC. The amino acids were measured by HPLC using the AccQTag method (Waters) according to the manufacturer's protocol. Briefly, the media collected after treatments were de-proteinized by adding 4X acetonitrile (by volume) ...
Furthermore, we have shown that key genes were gained before LVCCA, and that LCCA also evolved through a reduction in pathways involved in de novo biosynthesis and hence a dependence on uptake of essential metabolites. Chlamydiae diverged from other PVC bacteria between 1 and 2 Ga, ...
Hops are valued for their secondary metabolites, including bitter acids, flavonoids, oils, and polyphenols, that impart flavor in beer. Previous studies have shown that hop yield and bitter acid content decline with increased temperatures and low-water s