Adams MD, Celniker SE, Holt RA, Evans CA, Gocayne JD, Amanatides PG, Scherer SE, Li PW, Hoskins RA, Galle RF, George RA, Lewis SE, Richards S, Ashburner M, Henderson SN, Sutton GG, Wortman JR, Yandell MD, Zhang Q, Chen LX, Brandon RC, Rogers YH, Blazej RG, Champe M, Pfei...
RNAs from cell lines were extracted with a modified version of this procedure using an RNAsol B kit (Biotecx, Houston, TX) or an Ultraspect RNA kit (Biotecx) according to the manufacturer’s protocol. Polyadenylated RNA was purified through two cycles of binding to and elution from an ...