The fact that interspecific gene flow is considerably higher in Middle Eastern than in other gray wolves may also explain the distinctness of this population. The structure between Northwestern and Eastern African golden wolves can be explained using a similar argument—the former have highest levels ...
by studying genes in the latter category, scientists can learn which genes or gene sets may be important in maintaining species integrity in the face of gene flow.
c Activation of 6 target genes by GNE-3 sgRNAs normalized to the activation efficiency of the same spacer sequence in a 2.0 format (dashed line). Normalized gene activation was evaluated in Zeocin selected populations by qRT-PCR (left) at day 14 post-sgRNA infection or by flow cytometry (...
Galicia is shown to be included in the Western-Central European cluster, together with other Spanish populations. When only considering populations from Mediterranean Europe, the Galician population revealed a degree of genetic flow similar to that of the majority of the populations from this ...
Gene flow among European populations of Dover sole (Solea solea, Teleostei) The Dover sole () is a commercially important flatfish inhabiting the continental flat of the warm-temperate parts of the northeast Atlantic and the Mediterranean Sea. Spawning takes place in spring at several spawning groun...
To assess inference of the cell cycle, we considered a mouse embryonic stem cell cycle dataset [36], where embryonic stem cells were collected along three stages of the cell cycle (G1, S, G2/M). Cell cycle phase was manually annotated a priori based on flow sorting cells according to ...
At least 4% of the transcriptome studied is being differentially expressed between ecotypes living in alternative habitats, even when gene flow is still substantial between ecotypes. We could identify a candidate transcript of such ecotype differentiation: Cytochrome c Oxidase Subunit I (COI), a mit...
The in vivo gene response associated with hyperthermia is poorly understood. Here, we perform a global, multiorgan characterization of the gene response to heat stress using an in vivo conscious rat model. We heated rats until implanted thermal probes in
Mutagenic NHEJ of this DSB could generate indels inducing “3n + 1”-bp frame-shift, thus reframing GFP to the correct frame and making the cell GFP+. GFP+ cells could be measured by flow cytometry. Therefore, the percentages of GFP+ cells at 72 h post-transfection could reflect the...
An aliquot of 10 μl of each tryptic digest was injected on a C18 peptide trap (Agilent Technologies) and desalted with 0.1% formic acid at a flow rate of 2 μl/min for 45 min. Peptides were eluted from the trap and separated on a reversed phase nano-HPLC column (PicoFritTM; 75 ...