Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is used to quantify RNA. RT-qPCR can be performed as a one-step or two-step procedure. The most common method for looking at gene expression is two-step RT-qPCR.
is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas ...
Gene Co-Expression Networks Gene expression is the process by which the information encoded in a gene is used to synthesize a gene product (i.e., a protein or RNA). Transcription is the first step of gene expression, in which the DNA of the gene is copied intoRNA.Gene co-expression net...
In the present work we used a custom-made microchip from Agilent with 60-mer probes for these ESTs, to determine the gene expression profile during intestinal regeneration. Here we compared the expression profile of animals at three different intestinal regeneration stages (3-, 7- and 14-days p...
cell culture by Stein, A.][Successful gene expression analysis by multiplex, real-time, one-step RT-PCR, irrespective of the targets amplifies by Engel... N Vol. - 《Biotechniques》 被引量: 0发表: 2007年 Coordinately up-regulated genes in ovarian cancer A better understanding of the molecu...
We generated annotation files in which SNPs are assigned to genes based on their association with gene expression. The SNP-gene associations are tissue specific; hence we can estimate what genes are more highly associated with a disease at the tissue level. Part 2 conducts eMAGMA gene-set ...
Therefore, we used human induced pluripotent stem cell derived intestinal epithelial cells, grown as a cell layer, to study the effects of butyrate, propionate and acetate on whole genome gene expression in the cells. For this, cells were exposed to concentrations of 1 and 10 mM of the ...
Multi-step screening of DNA/lipid nanoparticles and co-delivery with siRNA to enhance and prolong gene expression Plasmid DNA offers extended transgene expression duration compared to mRNA technologies. Here, using a multi-step screening platform, the authors report the best performing nanoparticle formul...
The fast growing field of molecular imaging has achieved major advances in imaging gene expression, an important element of gene therapy. Gene expression i
step is usually a background correction, which filters out the fluorescence noise obtained by nonspecific hybridization of mRNA. In addition, it is also required to compensate for the artificial variability on expression signal. This variability can be due to the amount of mRNA in different samples...