GeneExpressionProfiling基因表达谱分析 系统标签: profilinggeneexpressioneditedprotocols基因表达 GeneExpressionProfilingMETHODSINMOLECULARBIOLOGY™JohnM.Walker,SERIESEDITOR286.TransgenicPlants:MethodsandProtocols,editedbyLeandroPeña,2004285.CellCycleControlandDysregulationProtocols:Cyclins,Cyclin-DependentKinases,andOtherFa...
2Gene Expression Profiling Gene expression profilingis a powerful tool to explore cellular states, development, and disease. Investigation of small-molecule mechanisms of action was among the first applications of gene expression profiling (Schena et al., 1996; Stockwell, Hardwick, Tong, & Schreibe...
Microarray analysis is a highly efficient tool for assessing the expression of a large number of genes simultaneously, and offers a new means to classify cancer and other diseases. Gene expression profiling can also be used to predict clinical outcome and response to specific therapeutic agents. ...
When reporting gene expression profiling studies, it is typical to report the genes that had significantly different expression profiles under the experimental conditions. This is limiting because: Cellular differentiation means different cel...
phosphate levels, and ambient pH shifts23. Gene expression profiling is an effective means of studying response to different environmental stimuli and a necessary component in identifying genes and regulatory mechanisms associated with biological processes in any organism. Choosing a proper reference gene ...
Gene expression fold-change differences in Combi and SARB hybrid after 24 h of treatment. Values represent means ± SEM (n = 3), (*p < 0.05; **p < 0.01; ***p < 0.001; One way-ANOVA). B2M was used as a housekeeping gene. (G) Western Blot analysis of ...
Microarray analysis is a highly efficient tool for assessing the expression of a large number of genes simultaneously, and offers a new means to classify cancer and other diseases. Gene expression profiling can also be used to predict clinical outcome and response to specific therapeutic agents. Thi...
There are multiple methods to perform each of the steps described (background correction, normalization, and summarization). In addition, preprocessing steps are platform-dependent, which means they need to be adjusted to the specific array technology. Since the final expression value can vary greatly...
Insights on PRAME and osteosarcoma by means of gene expression profiling.Toledo SR;Zago MA;Oliveira ID;et al
This means that some genes with significantly low raw p-values which is the case for TNF-α and IL-6 will be deemed non differentially expressed after adjusting for multiple testing although the expression profile of TNF-α is very similar to that observed by Q-PCR. Also the two ...