coli clone that, when ingested by the macrophages, allowed generation of the appropriate peptide/MHC class II complex and T cell activation. We show that the antigenic peptide is derived from a previously unknown listeria gene product with characteristics of a membrane-bound protein....
Therefore, the cloning and expression of these clusters is significantly more challen- ging than cloning of a single gene and expressing its product.61 Also, these clusters/genes may not be expressed in heterologous hosts due to the lack of signals naturally present in their native strains. This...
and principles, which culminate in a detailed process rationale and, ultimately, the experimental techniques themselves. While many lab manuals list background references, this innovative book combines both theory and procedure into one manageable volume. In addition to this theoretical background ...
the seventh edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are ...
John F. Warner, in Principles of Tissue Engineering (Second Edition), 2000 GENE DELIVERY Although the various viral- and plasmid-based gene delivery vehicles differ in composition and function, their ultimate goal is the production of a therapeutic protein in sufficient quantity at the appropriate ...
design for pulmonary therapy. Secondly, we outline the application of nanomaterial-assisted gene editing and synthetic biology in developing novel therapies for pulmonary diseases. Finally, we propose the prospects for utilizing these techniques for lung disease treatment and their possible clinical ...
Cloning of sgRNAs in multi-guide expression vectors Multi-guide plasmids for CRISPRi were cloned as previously described36. Briefly, four different sgRNAs were cloned into the sgRNA expression plasmid SP19936, with Golden Gate cloning, such that each sgRNA is controlled by a different Pol III pr...
Re-engineering biosynthetic assembly lines, including nonribosomal peptide synthetases (NRPS) and related megasynthase enzymes, is a powerful route to new antibiotics and other bioactive natural products that are too complex for chemical synthesis. Howev
Systematic mapping of nuclear domain-associated transcripts reveals speckles and lamina as hubs of functionally distinct retained introns Molecular Cell, Volume 82, Issue 5, 3 March 2022, Pages 1035-1052.e9 A. Rasim Barutcu, Mingkun Wu, Ulrich Braunschweig, Boris J.A. Dyakov, Zheng Luo, Kyle ...