Liu Z, Kanzawa N, Ono S (2011) Calcium-sensitive activity and conformation of Caenorhabditis elegans gelsolin-like protein 1 are altered by mutations in the first gelsolin-like domain. J Biol Chem 286: 34051-34059. doi: 10.1074/jbc.M111.237404 PMID: 21840993...
Villin is an actin-binding protein of relative molecular mass (Mr) 95,000 found in the core bundle of microfilaments in brush border microvilli from intestine1–3. In physiological calcium concentrations (<1 µM), villin crosslinks actin filaments i
Gelsolin is a 80 kDa protein displaying six homologous, gelsolin-like, domains (named G1 to G6) existing as two alternative splicing variants: a 24-residue shorter intracellular form and a variant secreted in the bloodstream1,2. Both isoforms bind actin fibrils, severing the filaments and ...
Calcium-sensitive Activity and Conformation of Caenorhabditis elegans Gelsolin-like Protein 1 Are Altered by Mutations in the First Gelsolin-like Domain. like protein-1 (GSNL-1) has only four gelsolin-like domains (G1-G4) and still exhibits Ca2+ -dependent actin-filament severing and capping ac...
binding site of G1. Finally, to tighten up the gelsolin–actin complex, Ca2+binds to the two type-1 sites shared between actin and gelsolin, which might explain the very high affinity of gelsolin to actin as recently demonstrated for plant villin, a gelsolin-like protein (Mierset al., ...
SAXS-based structure reconstructions suggested a pH-activated "open" state of gelsolin in which G1 is detached from domain G3 and domains G2-G6 still retain the inactive-like structure. This state is 1Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, ...
We would like to thank the HANDLS clinical staff and participants, Dr. Dimitrios Kapogiannis for providing access to the NanoSight and Althaf Lohani for technical support. Funding This work was supported by the National Institute on Aging Intramural Research Program project numbers AG000513 and AG...
Cadmium (Cd2+) is known to cause a selective disruption of the filamentous actin cytoskeleton in the smooth muscle-like renal mesangial cell. We examined t... MD Apostolova,Tania Christova and Douglas M. Templeton - 《Toxicological Sciences》 被引量: 44发表: 2006年 ...
Six-domain gelsolin regulates actin structural dynamics through its abilities to sever, cap and uncap F-actin. These activities are modulated by various cellular parameters like Ca2+and pH. Until now, only the molecular activation mechanism of gelsolin by Ca2+has been understood relatively well. The...
Addition of a small C-terminal F-actin binding ‘headpiece’ domain converts a gelsolin-like molecule into a bundling protein, termed ‘villin’ based on its localization in microvilli [22], [23]. And, substitution of a handful of residues in domain G1 appears to be key for distinguishing...