using an iScript Advanced cDNA Synthesis Kit (Bio-Rad). Downstream qPCR amplification was performed using iQ SYBR Green Supermix (Bio-Rad) in an CFX Connect Real-Time PCR System (Bio
The prepurified samples were then spiked with a known amount of a recombinant deletion mutant of human MMP-9 that does not interfere with any mouse gelatinolytic enzyme. The spiking reference was included for quantitative standardization. In addition, to identify and qualitatively distinguish between...