Denaturing Gradient Gel Electrophoresis of β-Thalassemia Samples on the DCodeTM SystemResults and DiscussionThe b-thalassemia samples were first run on perpendicularDGGE gels to determine the optimum concentrationof denaturant to use with CDGE. Figure 1 shows theheterozygous mutant IVS1-6 run on ...
Gel electrophoresis is an accessible, widely applicable and mature protein resolving technology. As the original top-down approach to proteomic analyses, among its many attributes the high resolution achievable by two dimensional gel-electrophoresis (2DE)1 ensures that it remains an effective analytical ...
RESULTS AND DISCUSSION We had previously shown that preincubation with the IEF-compatible zwitterionic detergent N-decyl-N,N′-dimethyl-3-ammonio-1-propanesulfonate for 10 min at room temperature prior to the addition of standard IPG buffer (see “Experimental Procedures”) improved isoelectric focusin...
Polyacrylamide gel electrophoresis (PAGE) coupled with mass spectrometry has been well established for separating, identifying and quantifying protein mixtures from cell lines, tissues or other biological samples. The copolymerization process of acrylami
The validation of OFFGEL electrophoresis on separation of eukaryote tryptic digests will permit to include it in proteomic workflow of complex samples. Results and discussion The OFFGEL fractionator system was used in order to separate secretome and depleted plasma peptides according to their pI ...
Four separate waste vials were used for the collection of 1 N NaOH, 0.1 N NaOH, Protein capillary conditioning solution and Protigel, respectively. Pro- tigel containing inlet and outlet home vials were exchanged every eight runs. Results and discussion Molecular weight determination The molecular...
Results and Discussion Databank Search Results Databank search results obtained from theE. coli2D gel samples are summarized in Table 1. Two different types of databank search were performed on the MS or PSD data obtained from each sample well. MS data w...
Results and discussion GelJ has been successfully tested on a battery of 65 images taken from agarose pulsed-field gel electrophoresis (PFGE)-gels — GelJ can also be applied without any special hindrance to gel-images obtained using other techniques such as RAPD, rep-PCR, AFLP, SSR and so...
4.6.4Vertical Pulse Field Gradient Electrophoresis This was designed and developed by Gardiner et al., using two oppositely positioned electrodes in such a manner that when electric field is switched DNA moves toward another set of electrodes, which results in a zigzag movement of DNA from the tr...
However, some of the most suitable primers were Results and discussion identical to the FISH probes suggested by Ashelford et al. (2002) — Alf28f, Beta359f and Beta682r. Other In silico analysis of the group-specific PCR primers probes suggested by Ashelford et al. (2002) or Blackwood ...