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DNA samples (∼10 μL) such as a standard lambda HindIII ladder (Fisher Scientific; ∼$1 per lane) or genomic extract were added 1:1 in loading dye (one drop corn syrup with three drops green food coloring) using a pipetter. Electrophoresis proceeded for 1 to 1.5 hr, while ...
Subsequently, the excess dye was washed away with distilled water. Nile Red and Nile Blue-A were excited at wavelengths of 633 and 488 nm, respectively, to obtain the emission spectra. Microscopic images of the original samples were obtained using a laser confocal microscope (Nikon A1R HD25, ...
dye or a nucleic acid, including deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), to deliver the drug or other biomolecule to cells in the body of an animal, including a mammal, including a human; form a vesicle encapsulating a drug or other biomolecule including a nucleic acid, inclu...
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Before reaction, the dye solution (50 mL) is prepared by mixing RhB and DI water in a molar ratio of 3.6 × 10−6 : 1 and stirred by a magnetic stirrer (Fisher Scientific, Republic of Korea) for 45 min in dark condition to ensure the proper adsorption-desorption equilibrium ...
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Methanol was initially used as a solvent/porogenic agent for the sol–gel material; however, it was replaced with DI water due to the dye solubility. 2.2.2. Gelation vs. Base Catalyst The amount of the base catalyst in the formulation was tested to allow time for deposition on the ...