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Thus, the smaller wells function as analytical features for analysis and the larger, bead-filled wells function as fiducials. As an alternative to wells the fiducial features can be channels, such as those present in the bulls-eye configuration exemplified above, and the channels can have ...
38,39 To handle low concentrations of proteins, protein adsorption on chromatographic media, such as C18 beads, has been used to concentrate the proteins from a dilute solution, followed by on-bead digestion using trypsin.40 This method reduces the extent of trypsin autolysis in analyzing low ...
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