GDP-d-mannose dehydratase (GMD) catalyzes the first step of the pathway that converts GDP-d-mannose to GDP-l-fucose in bacteria, plants and mammals. Recently, the gene coding for GMD has been identified and sequenced in E. coli. Based on this sequence, we have expressed and purified GMD...
The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C 5 hydrogen has been established using GDP- d-[5- 3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of 3H with the aqueous ...
D1.GDP-mannose4,6-dehydratase(GMD)assaydevelopmentandpHrateprofilesThisprojectwillenablestudentstofunctionascompetentenzymologiststhroughthedevelopmentofa..
GDP-D-mannose dehydratase 英文别名: GDP-D-mannose dehydratase 中文名: 中文别名: CBNumber: CB51345372 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商0 GDP-D-mannose dehydratase化学性质 安全信息 GDP-D-mannose dehydratase性质、用途与生产工艺 ...
alpina grown on GMD inhibitors medium, which suggests that GDP-L-fucose is functionally significant in lipid metabolism. CONCLUSIONS: This is the first time that an isofunctional GDP-D-mannose-4,6-dehydratase has been characterized in a microorganism. 展开 ...
This work reports the biochemical and functional analysis of the Burkholderia cenocepacia J2315 bceN gene, encoding a protein with GDP-D-mannose 4,6-dehydratase enzyme activity (E.C.4.2.1.47). Data presented indicate that the protein is active when in the tetrameric form, catalyzing the convers...
In all species examined, GDP-fucose is synthesized from GDP-mannose in a three-step reaction catalyzed by two enzymes, GDP-mannose 4,6 dehydratase and a du... WS Somers,ML Stahl,FX Sullivan - 《Structure》 被引量: 148发表: 1998年 Barber, G. A. Observations on the mechanisms of the ...
Molecular Cloning of Human GDP-mannose 4,6-Dehydratase and Reconstitution of GDP-fucose Biosynthesis in Vitro We have cloned the cDNA encoding human GDP-mannose 4,6-dehydratase, the first enzyme in the pathway converting GDP-mannose to GDP-fucose. The message is ex... Sullivan,X F. - 《...
CONCLUSIONS: This is the first time that an isofunctional GDP-D-mannose-4,6-dehydratase has been characterized in a microorganism.doi:10.1007/s10529-016-2153-9Wang, HongchaoZhang, ChenChen, HaiqinYang, QinZhou, XinGu, ZhennanZhang, Hao
6-dehydrataseGDP-L-fucoseIsoformMortierella alpinaTo clarify the molecular mechanism of GDP-l-fucose biosynthesis in Mortierella alpina.Wang, HongchaoZhang, ChenChen, HaiqinYang, QinZhou, XinGu, ZhennanZhang, HaoChen, WeiChen, Yong Q.Biotechnology Letters...