Fission yeast Cdc42p, a small GTPase of the Rho family, is essential for cell proliferation and maintenance of the rod-like cell morphology. Scd1/Ral1p is a GDP-GTP exchange factor (GEF) for Cdc42p. This study and a parallel study by others establish that Gef1p is another GET for Cdc...
Guanosine triphosphate (GTP) spontaneously binds to Gα, resulting in the dissociation of the GTP-bound form of Gα (Gα-GTP) and Gβγ. Subsequently, both Gα-GTP and Gβγ bind to the effector proteins and regulate their functions, leading to cellular responses. The G protein returns ...
Structural comparisons reveal that binding to GTP or GDP-mannose induces significant conformational changes primarily for the loop regions surrounding the active site, and results in an overall more compact conformation (Supplementary Fig.S8c). Indeed, the key lysine residue (K170) in the F(V)EKP...
Filamenting temperature-sensitive mutant Z (FtsZ) is an essential cell division protein, a eukaryotic tubulin homologue and a promising drug target. During cytokinesis, FtsZ polymerises in the presence of GTP to form Z-ring and recruits other proteins at this site that eventually lead to the ...
Gα proteins bind quiescent GPCRs in an inactive GDP-bound form that associates in a complex with Gβ and Gγ subunits. However, upon activation of GPCRs through chemical or physical stimulation, the Gα subunit undergoes a GDP to GTP exchange that causes the Gα/β/γ heterotrimer to ...
Rab proteins form a large family of small GTP-binding proteins with a wide range of functions in the coordination of vesiculo-tubular traffic (1), which include directional tethering, docking and fusion of vesicles to target membranes (2), vesicle motility (3), and cargo recruitment (4). Th...
The 3014-A kit is formatted for 96-well plates while the 3014-1K and 3014- 10K kits are for 384-well and higher densities. Able to accommodate GTP concentrations ranging from 0.1 to 1,000 μM, the Transcreener® GDP FI Assay provides excellent data quality (Z′ = 0.7) at low ...
SAXSandX-rayCrystallographySuggestanUnfolding ModelfortheGDP/GTPConformationalSwitchofthe SmallGTPaseArf6 ValérieBiou 1 ⁎,KaheinaAizel 1 ,PierreRoblin 2 ,AurélienThureau 3 , EricJacquet 3,4 ,SebastianHansson 1 ,BernardGuibert 1 , EricGuittet 3 ,CarinevanHeijenoort 3 ,MahelZeghouf 1 ,Javier...
The Sec7 domain of the guanine nucleotide exchange factor ARNO (ARNO-Sec7) is responsible for the exchange activity on the small GTP-binding protein ARF1. ARNO-Sec7 forms a stable complex with the nucleotide-free form of [17]ARF1, a soluble truncated form of ARF1. The crystal structure ...
Discussion Previously, we have shown that Rab5 and Rab7 regulate the endocytic pathway in Leishmania, whereas the con- ventional secretory pathway in the parasites is regulated by its Rab1 homologue20,21,27. We have also shown that GTP form of the respective Rabs are present on appropriate ...