Filamenting temperature-sensitive mutant Z (FtsZ) is an essential cell division protein, a eukaryotic tubulin homologue and a promising drug target. During cytokinesis, FtsZ polymerises in the presence of GTP to form Z-ring and recruits other proteins at this site that eventually lead to the ...
The 3014-A kit is formatted for 96-well plates while the 3014-1K and 3014- 10K kits are for 384-well and higher densities. Able to accommodate GTP concentrations ranging from 0.1 to 1,000 μM, the Transcreener® GDP FI Assay provides excellent data quality (Z′ = 0.7) at low substr...
Structural comparisons reveal that binding to GTP or GDP-mannose induces significant conformational changes primarily for the loop regions surrounding the active site, and results in an overall more compact conformation (Supplementary Fig.S8c). Indeed, the key lysine residue (K170) in the F(V)EKP...
Second, we detected a Rab5-specific GDP/GTP exchange activity on CCV, which required the presence of the Rab5-RabGDI complex for maximal stimulation. These results demonstrate that Rab5 can bind to transport vesicles in vitro and that CCV contain one or more factors that promote release of...
The kinetics of the interaction of Rab7 with REP-1 have been investigated using the fluorescence of GDP and GTP analogs at the active site of Rab7. The results show that REP-1 has higher affinity for the GDP bound form of Rab7 (Kd=1 nM) than for the GTP bound form (Kd=20 nM). Bo...
Guanosine triphosphate (GTP) spontaneously binds to Gα, resulting in the dissociation of the GTP-bound form of Gα (Gα-GTP) and Gβγ. Subsequently, both Gα-GTP and Gβγ bind to the effector proteins and regulate their functions, leading to cellular responses. The G protein returns ...
gdp软件介绍(IntroductiontoGDPsoftware)IntroductiontotheusageofGTPsoftwareGuitar-proisaverypopularnetworksoftware,almosteveryguitarsiteprovidesguitarspectrumhasGTPformat.Andtheinterfaceisveryintuitivetosixlinespectrumasthemainformofinput,aslongasyouarefamiliarwiththepaper'sguitarspectrum,GPismorehandy.Forbeginners,aslon...
Fission yeast Cdc42p, a small GTPase of the Rho family, is essential for cell proliferation and maintenance of the rod-like cell morphology. Scd1/Ral1p is a GDP-GTP exchange factor (GEF) for Cdc42p. This study and a parallel study by others establish that Gef1p is another GET for Cdc...
Properties of the catalytic domain of CDC25, a Saccharomyces cerevisiae GDP/GTP exchange factor: comparison of its activity on full-length and C-terminal t... Two C-terminal fragments (334 and 509 amino acid residues) of CDC25, a Saccharomyces cerevisiae GDP/GTP exchange factor, and the RAS...
receivedinrevisedform29July2010;accepted2August2010Availableonline13August2010EditedbyK.MorikawaKeywords:SmallGTPases;Arf;Crystalstructure;SAXS;NMRThesmallGTPasesArf1andArf6havenonoverlappingfunctionsincellulartrafficdespitetheirveryhighsequenceandstructuralresemblance.Notably,theexquisiteisoformspecificityoftheirguanine...