在GCRV-II中发现的S6、S7和S11基因分别编码VP4、VP56和VP35,此三种关键外衣壳蛋白具有强大的免疫原性,可以作为抗原候选物,在宿主体内引发免疫反应,在病毒组装和细胞侵袭中起着关键作用。研究人员分别通过大肠杆菌表达系统和酵母表达系统将三种蛋白进行表达纯化并进行组装,pull down实验结果表明,三个蛋白在特定的组装缓...
在GCRV-II中发现的S6、S7和S11基因分别编码VP4、VP56和VP35,此三种关键外衣壳蛋白具有强大的免疫原性,可以作为抗原候选物,在宿主体内引发免疫反应,在病毒组装和细胞侵袭中起着关键作用。研究人员分别通过大肠杆菌表达系统和酵母表达系统将三种蛋白进行表达纯化并进行组装,pull down实验结果表明,三个蛋白在特定的组装缓...
The IgM gene expression in the SWCNTs-pcDNA-vp4-3 immune group (5 渭g per fish) was 3.14 times as much as control group. The relative percentage survival in SWCNTs-pcDNA-vp4-3 immune group (5 渭g per fish) reached the peak, protected 74% of grass carp from type II GCRV. ...
The IgM gene expression in the SWCNTs-pcDNA-vp4-3 immune group (5 mu g per fish) was 3.14 times as much as control group. The relative percentage survival in SWCNTs-pcDNA-vp4-3 immune group (5 mu g per fish) reached the peak, protected 74% of grass carp from type II GCRV. ...
VP4, VP56, and VP35. In this study, VP4, VP56, and VP35 were expressed in anEscherichia coliexpression system and aPichia pastorisexpression system. The successful assembly of uniform, stable, and non-toxic VP4/VP56/VP35 VLPs was confirmed through various assays. After vaccination and G...
2.2. Screening of Surface Fibrin VP56 Epitope of GCRV The complete sequence of VP56 protein (GenBank accession number: MK675081.1) was analyzed by DNASTAR software, and divided into four antigen fragments and named them: VP56-1, VP56-2, VP56-3 and VP56-4. The VP56 full sequence, fo...