DBP binds with high affinity 85–90 % of circulating 25(OH)D, albumin binds with low affinity 10–15 % of circulating 25(OH)D and less than 1 % of 25(OH)D is in the free form (Bikle et al. 1986). The main function of DBP is to stabilize and prolong the half-life of 25(...
(1988). SNPs were genotyped using a Se- quenomÒ platform (San Diego, California) and the iPLEX Gold reaction. The SNPs and the primers used are listed in Supplementary Table 1. Each PCR reaction contained 10 ng genomic DNA, 0.5 U HotStart Taq (Qiagen), 1.25 9 Enzyme Buffer (Qiagen)...
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